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首页> 外文期刊>Microbial Pathogenesis >Mild changes in the mucosal microbiome during terminal ileum inflammation
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Mild changes in the mucosal microbiome during terminal ileum inflammation

机译：粘膜微生物末端炎症期间的温和变化

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Patients with inflammation in the terminal ileum have high morbidity. In genetically susceptible hosts, chronic intestinal inflammation targeting the resident intestinal microbiota develops, but the microbial signature of the terminal ileum is poorly studied. To improve understanding of the mechanisms underlying the high prevalence of terminal ileum inflammation, we used 16S rRNA sequencing to analyse the mucosa-associated microbiota of the terminal ileum under intestinal homeostasis and inflammation conditions. Mucosal biopsy is the most commonly used sampling technique for assessing microbial communities associated with the intestinal mucosa. Thirty patients (15 with terminal ileum inflammation and 15 controls) underwent colonoscopy and biopsies were taken from the terminal ileum. Diagnosis depended on a combination of endoscopic and histological factors. To determine the composition and diversity of the microbiota, the 16S rRNA was analysed, and a variety of bioinformatics analyses were performed. Among the patients, composition analysis showed that the most abundant phyla identified in the terminal ileum samples were Fusobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria. At the phylum level, the relative proportion of Bacteroidetes was lower in patients with inflammation than in control patients. In addition, there was an increase in the abundance of the phyla Proteobacteria and Lentisphaerae in patients with inflammation. The abundances of the dominant microbes in the terminal ileum were not significantly different between patients in an inflammatory state and controls. These results confirm that partial dysbiosis of the intestinal mucosa-associated microbiota composition is associated with terminal ileum inflammation.
机译：终末末端炎症患者患有高发病率。在遗传易感宿主中，患者靶向植物肠道微生物群的慢性肠炎症发生，但研究末端的微生物特征是较差的。为了改善对末端炎症末端炎症的高患病率的理解，我们使用16S RRNA测序在肠道稳态和炎症条件下分析末端回肠的粘膜相关微生物。粘膜活检是评估与肠粘膜相关的微生物社区最常用的采样技术。三十名患者（15末末末端炎症和15个对照）从末端感应术后进行结肠镜检查和活组织检查。诊断取决于内窥镜和组织学系的组合。为了确定微生物群的组成和多样性，分析了16S rRNA，进行了各种生物信息学分析。在患者中，组成分析表明，终端回肠样品中鉴定的最丰富的Phyla是Fusobacteria，植物菌，常规，菌斑和抗菌菌。在门，炎症的炎症患者的炎症患者的相对比例低于对照患者。此外，炎症患者的Phyla Proteobacteria和Lentisphaerae的丰度有所增加。炎症状态和对照组患者之间的末端Hileum中显性微生物的丰度在患者之间没有显着差异。这些结果证实，肠粘膜相关的微生物群组合物的部分脱粘与末端感染症相关。

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