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首页> 外文期刊>Micron: The international research and review journal for microscopy >Identification of collagen fibrils in cross sections of bone by electron energy loss spectroscopy (EELS)
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Identification of collagen fibrils in cross sections of bone by electron energy loss spectroscopy (EELS)

机译：电子能损谱（EELS）鉴定骨横截面骨胶原纤维

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Transmission electron microscopic (TEM) images of ion-milled bovid cortical bone cut approximately normal to the axes of fibrils show that mineral occurs in the form of plates surrounding and laying between circular or elliptical features about 50 nm in diameter. The classification of these features as either pores or collagen fibrils is highly debated. Electron energy loss spectroscopy (EELS) mapping of these features in ion milled sections shows that they are lacking significant amounts of mineral or collagen, although their appearance suggests that they are cross sections of collagen fibrils. However, analogous sections prepared using an ultramicrotome show that, while these circular features show reduced concentrations of calcium and phosphorus, some of them contain quantities of carbon and nitrogen in bonding states comparable to the composition of collagen. This work demonstrates that the observed circular features are sections of collagen fibrils, but that bombardment by argon ions during broad beam ion milling destroys the collagen and associated gap-zone mineral.
机译：离子铣削肉豆皮质骨的透射电子显微镜（TEM）图像大约正常到原纤维的轴上，表明矿物在围绕圆形或椭圆形特征之间的圆形或直径约50nm之间铺设的矿物。这些特征的分类是毛孔或胶原型原纤维的争论。离子铣削部分中这些特征的电子能量损失光谱（EEL）映射表明它们缺乏大量的矿物或胶原蛋白，尽管它们的外观表明它们是胶原型原纤维的横截面。然而，使用超微网上运动方案制备的类似部分表明，虽然这些圆形特征显示出降低的钙和磷的浓度，但其中一些含有与胶原蛋白组成相当的粘合状态中的碳和氮。这项工作表明，观察到的圆形特征是胶原型原纤维的切片，但是宽梁离子铣削期间的氩离子轰炸破坏了胶原蛋白和相关间隙区矿物质。

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