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The configuration of DMD and the maximum intensity projection method for improving contrast in DMD-based confocal microscope

机译:DMD的配置和最大强度投影方法,用于改进DMD基共聚焦显微镜对比度

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摘要

In this article, an operation strategy of digital micromirror device (DMD) and the maximum intensity projection (MIP) image processing method are proposed to improve the contrast of images in confocal microscopy. First, the configuration of DMD is demonstrated and the effect of scanning unit size on image performance is analyzed. Then, the image processing method MIP is applied. According to the MIP method, only the maximum intensity projection point of the same pixel is chosen from every image, and the maximum intensity projection point exactly corresponds to the positon where mirror is at on position during the scanning process in DMD-based confocal microscope system,. Thus, high contrast of images can be achieved by using MIP. Finally, experiments are conducted to verify imaging performance by changing the parameter of scanning unit size and applying a MIP image processing technique. The results show that DMD scanning unit size and MIP image processing techniques play important roles in improving image contrast. Smaller scanning unit size of DMD improves axial contrast but greatly decreases the signal to noise ratio, which thus leads to reduced image contrast. Larger scanning unit size produces a better signal to noise ratio, thus better image contrast. However, a large S will sacrifice the processing time. Therefore, DMD scanning unit size should be smaller on the premise that image contrast can be satisfied.
机译:在本文中,提出了一种数字微镜器件(DMD)的操作策略和最大强度投影(MIP)图像处理方法,以改善共聚焦显微镜中的图像的对比度。首先,分析了DMD的配置,分析了扫描单元尺寸对图像性能的影响。然后,应用图像处理方法MIP。根据MIP方法,仅从每个图像中选择相同像素的最大强度投影点,并且最大强度投影点与镜像在基于DMD的共聚焦显微镜系统中的扫描过程期间镜像处于接通位置的位置。 。因此,可以通过使用MIP来实现图像的高对比度。最后,通过改变扫描单元尺寸的参数和应用MIP图像处理技术来进行实验以验证成像性能。结果表明,DMD扫描单元尺寸和MIP图像处理技术在提高图像对比度方面发挥着重要作用。 DMD的较小扫描单元尺寸提高了轴向对比度,但大大降低了信噪比,从而导致图像对比度降低。较大的扫描单元尺寸会产生更好的信噪比,从而更好的图像对比度。但是,大型S会牺牲处理时间。因此,DMD扫描单元大小在图像对比度的前提下应更小。

著录项

  • 来源
    《Microscopy research and technique》 |2018年第9期|共7页
  • 作者单位

    Univ Shanghai Sci &

    Technol Coll Opt Elect &

    Comp Engn Shanghai Key Lab Contemporary Opt Syst 516 Jungong Rd Shanghai 200093 Peoples R China;

    Univ Shanghai Sci &

    Technol Coll Opt Elect &

    Comp Engn Shanghai Key Lab Contemporary Opt Syst 516 Jungong Rd Shanghai 200093 Peoples R China;

    Univ Shanghai Sci &

    Technol Coll Opt Elect &

    Comp Engn Shanghai Key Lab Contemporary Opt Syst 516 Jungong Rd Shanghai 200093 Peoples R China;

    Univ Shanghai Sci &

    Technol Coll Opt Elect &

    Comp Engn Shanghai Key Lab Contemporary Opt Syst 516 Jungong Rd Shanghai 200093 Peoples R China;

    Univ Shanghai Sci &

    Technol Coll Opt Elect &

    Comp Engn Shanghai Key Lab Contemporary Opt Syst 516 Jungong Rd Shanghai 200093 Peoples R China;

    Penn State Univ Dept Engn Sci &

    Mech 227 Hammond Bldg University Pk PA 16802 USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 显微镜;
  • 关键词

    digital micromirror device (DMD); image contrast; scanning unit size; the maximum intensity projection image processing (MIP);

    机译:数字微镜器件(DMD);图像对比度;扫描单元尺寸;最大强度投影图像处理(MIP);

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