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首页> 外文期刊>International Journal of Vegetable Science >Nitrate Content and Expression of Putative Nitrate Transporter Genes in Lettuce Fertilized with Nitrogen Fertilizers
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Nitrate Content and Expression of Putative Nitrate Transporter Genes in Lettuce Fertilized with Nitrogen Fertilizers

机译：用氮肥施用植物施用硝酸盐转运基因的硝酸盐含量及表达

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摘要

Nitrate accumulation in plants is influenced by environmental conditions and genetic variability. It is important to develop approaches that could be used to reduce nitrate levels in lettuce {Lactuca sativa L.) because nitrate accumulated in leaves can cause harmful health effects in humans. A greenhouse experiment was conducted to determine effects of ammonium nitrate or urea nitrogen on lettuce fresh weight, nitrate content, and characterization of nitrate transporter genes in lettuce. Head fresh weight in cultivars grown with the nitrogen fertilizers differed. All cultivars accumulated more nitrates with urea than with ammonium nitrate fertilizer. 'Great Lakes' had lower nitrate accumulation than other cultivars due to urea or ammonium nitrate treatment. Lettuce nitrate transporter genes LsNRTI and LsNRT2 were detected and their expression level varied due to cultivar and leaf position. 'Great Lakes' had low mRNA levels of LsNRTI and LsNRT2 in leaves compared to other cultivars. 'Great Lakes' could be used for lettuce production due to high yield and lower nitrate content.
机译：植物中的硝酸盐积聚受环境条件和遗传变异性的影响。重要的是开发可用于将硝酸盐水平降低莴苣（Lactuca Sativa L.）的方法，因为叶片中累积的硝酸盐会导致人类的有害健康影响。进行了温室实验，以确定硝酸铵或尿素氮对莴苣中硝酸盐的新鲜重量，硝酸盐含量，硝酸盐含量的影响。用氮肥生长的品种头部新鲜重量不同。所有品种含有比硝酸铵肥料更多的硝酸盐。由于尿素或硝酸铵处理，“大湖泊”的硝酸盐积累量低于其他品种。检测到莴苣硝酸盐转运蛋白LSNRTI和LSNRT2，并且其表达水平因品种和叶子位置而变化。与其他品种相比，“大湖泊”的LSNRTI和LSNRT2的LSNRTI和LSNRT2的较低程度低。由于高产率和硝酸盐含量降低，“大湖泊”可用于莴苣生产。

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  • 作者

    N. Razgallah; G. Abid; H. Chikh-Rouhou; A. Hassen; M. Mhamdi;

  • 作者单位

    University of Sousse Higher Agronomic Institute of Chott Mariem Laboratory of Vegetable Crops Chott Mariem Sousse Tunisia;

    University of Tunis El Manar Center of Biotechnology of Borj Cedria Laboratory of Legumes Hammam-Lif Tunisia;

    University of Sousse Regional Research Center of Horticulture and Organic Agriculture Laboratory of Horticulture Chott Mariem Tunisia;

    Center of Research and Water Technologies Laboratory of Wastewater Treatment Soliman Tunisia;

    University of Sousse Higher Agronomic Institute of Chott Mariem Laboratory of Vegetable Crops Chott Mariem Sousse Tunisia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 蔬菜园艺;
  • 关键词

    Lactuca sativa; gene expression analysis; nitrate;

    机译：Lactuca sativa;基因表达分析;硝酸盐;

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