Structural investigations of E-Coli dihydrolipoamide dehydrogenase in solution: Small-angle X-ray scattering and molecular docking

Dadinova L. A.; Rodina E. V.; Vorobyeva N. N.; Kurilova S. A.; Nazarova T. I.; Shtykova E. V.

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Structural investigations of E-Coli dihydrolipoamide dehydrogenase in solution: Small-angle X-ray scattering and molecular docking

机译：溶液中E-Coli二氢脂酰胺脱氢酶的结构研究：小角度X射线散射和分子对接

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Dihydrolipoamide dehydrogenase from Escherichia coli (LpD) is a bacterial enzyme that is involved in the central metabolism and shared in common between the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes. In the crystal structure, E. coli LpD is known to exist as a dimer. The present work is focused on analyzing the solution structure of LpD by small-angle X-ray scattering, molecular docking, and analytical ultracentrifugation. It was shown that in solution LpD exists as an equilibrium mixture of a dimer and a tetramer. The presence of oligomeric forms is determined by the multifunctionality of LpD in the cell, in particular, the required stoichiometry in the complexes.

机译：来自大肠杆菌（LpD）的二氢脂酰胺脱氢酶是一种细菌酶，参与中央代谢，并且在丙酮酸脱氢酶和2-氧戊二酸脱氢酶复合物之间共享。在晶体结构中，已知大肠杆菌LpD以二聚体形式存在。目前的工作集中在通过小角度X射线散射，分子对接和分析超离心来分析LpD的溶液结构。结果表明，LpD在溶液中以二聚物和四聚物的平衡混合物形式存在。寡聚形式的存在取决于细胞中LpD的多功能性，特别是复合物中所需的化学计量。

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《Crystallography reports》 |2016年第3期|共7页
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Dadinova L. A.; Rodina E. V.; Vorobyeva N. N.; Kurilova S. A.; Nazarova T. I.; Shtykova E. V.;
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1. Structural investigations of E-Coli dihydrolipoamide dehydrogenase in solution: Small-angle X-ray scattering and molecular docking [J] . Dadinova L. A., Rodina E. V., Vorobyeva N. N., Crystallography reports . 2016,第3期

机译：溶液中E-Coli二氢脂酰胺脱氢酶的结构研究：小角度X射线散射和分子对接
2. Structural characterization of lactate dehydrogenase dissociation under high pressure studied by synchrotron high-pressure small-angle X-ray scattering. [J] . Fujisawa T, Kato M, Inoko Y Biochemistry . 1999,第20期

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5. Application of Advanced X-Ray Scattering Techniques to Structural Investigations of Molecular Packing in Conducting Polymers [D] . Balliet, Courtney Lyn 2012

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机译：1SEP-02蛋白质结构波动由小角度X射线溶液散射和分子动力学模拟（1SEP综合结构生物学和生物分子露萨姆，研讨会，日本生物物理学会的第51次年会）

Structural investigations of E-Coli dihydrolipoamide dehydrogenase in solution: Small-angle X-ray scattering and molecular docking

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