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首页> 外文期刊>Mitochondrial DNA, Part A >The novel primers for mammal species identification-based mitochondrial cytochrome b sequence: implication for reserved wild animals in Thailand and endangered mammal species in Southeast Asia
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The novel primers for mammal species identification-based mitochondrial cytochrome b sequence: implication for reserved wild animals in Thailand and endangered mammal species in Southeast Asia

机译：哺乳动物物种鉴定线粒体细胞色素B序列的新型引物：泰国保留野生动物的对东南亚濒危哺乳动物物种的影响

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摘要

We presented the powerful techniques for species identification using the short amplicon of mitochondrial cytochrome b gene sequence. Two faecal samples and one single hair sample of the Asian tapir were tested using the new cytochrome b primers. Theresults showed a high sequence similarity with the mainland Asian tapir group. The comparative sequence analysis of the reserved wild mammals in Thailand and the other endangered mammal species from Southeast Asia comprehensibly verified the potential ofour novel primers. The forward and reverse primers were 94.2 and 93.2%, respectively, by the average value of the sequence identity among 77 species sequences, and the overall mean distance was 35.9%. This development technique could provide rapid, simple, and reliable tools for species confirmation. Especially, it could recognize the problematic biological specimens contained less DNA material from illegal products and assist with wildlife crime investigation of threatened species and related forensiccasework.
机译：我们使用线粒体细胞色素B基因序列的短扩增介绍了物种鉴定的强大技术。使用新的细胞色素B引物测试两种粪便样本和亚洲Tap的一个头发样品。结果表明，与大陆亚洲的Tap族的高序列相似之处。泰国保留野生哺乳动物的比较序列分析与东南亚其他濒临灭绝的哺乳动物物种综合地验证了我们的新底漆的潜在。通过77种序列之间的序列同一性的平均值分别为94.2和93.2％，总平均距离为35.9％。这种开发技术可以为物种确认提供快速，简单，可靠的工具。特别是，它可以识别出现问题的生物标本从非法产品中含有较少的DNA材料，并协助威胁物种的野生动物犯罪调查和相关的伪装箱。

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