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首页> 外文期刊>Molecular & cellular proteomics: MCP >Conodipine-P1-3, the First Phospholipases A(2) Characterized from Injected Cone Snail Venom
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Conodipine-P1-3, the First Phospholipases A(2) Characterized from Injected Cone Snail Venom

机译：Conodipine-p1-3，第一种磷脂酶A（2），其特征来自注射锥蜗牛毒液

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摘要

The phospholipase A(2) (PLA(2)s) superfamily are ubiquitous small enzymes that catalyze the hydrolysis of phospholipids at the sn-2 ester bond. PLA(2)s in the venom of cone snails (conodipines, Cdpi) are composed of two chains termed as alpha and beta subunits. Conodipines are categorized within the group IX of PLA(2)s. Here we describe the purification and biochemical characterization of three conodipines (Cdpi-P1, -P2 and -P3) isolated from the injected venom of Conus purpurascens. Using proteomics methods, we determined the full sequences of all three conodipines. Conodipine-P1-3 have conserved consensus catalytic domain residues, including the Asp/ His dyad. Additionally, these enzymes are expressed as a mixture of proline hydroxylated isoforms. The activities of the native Conodipine-Ps were evaluated by conventional colorimetric and by MS-based methods, which provide the first detailed cone snail venom conodipine activity monitored by mass spectrometry. Conodipines can have medicinal applications such inhibition of cancer proliferation, bacterial and viral infections among others. Molecular & Cellular Proteomics 18: 876-891, 2019. DOI: 10.1074/ mcp. RA118.000972.
机译：磷脂酶A（2）（PLA（2））超家族是普遍存在的小酶，其催化Sn-2酯键在Sn-2酯键处的水解。 PLA（2）在锥形蜗牛（Cododipines，CDPI）中的毒液由两条链组成，称为α和β亚基。透明素线分类为PLA（2）秒的第IX组内。在这里，我们描述了从康明斯毒素的注射毒液中分离的三个核细胞（CDPI-P1，-P2和-P3）的纯化和生化表征。使用蛋白质组学方法，我们确定了所有三个透明石的完整序列。 Conodipine-P1-3具有保守的共有催化结构域残基，包括ASP /他的二元。另外，这些酶表示为脯氨酸羟基化同种型的混合物。通过常规的比色测量和基于MS的方法评估本地芋头-SPS的活性，其提供由质谱法监测的第一个详细的锥形蜗牛毒液糖蛋白活性。迦图伊对药物可以具有这种抑制癌细胞的抑制，细菌和病毒感染等。分子和细胞蛋白质组学18：876-891,2017。DOI：10.1074 / MCP。 ra118.000972。

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    Moller Carolina; Davis W. Clay; Clark Evan; DeCaprio Anthony; Mari Frank;

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    Natl Inst Stand &

    Technol Marine Biochem Sci Chem Sci Div 331 Ft Johnson Rd Charleston SC;

    Natl Inst Stand &

    Technol Marine Biochem Sci Chem Sci Div 331 Ft Johnson Rd Charleston SC;

    Florida Atlantic Univ Dept Biomed Sci Boca Raton FL 33431 USA;

    Florida Int Univ Dept Chem &

    Biochem SW 8th St Miami FL 33119 USA;

    Natl Inst Stand &

    Technol Marine Biochem Sci Chem Sci Div 331 Ft Johnson Rd Charleston SC;

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  • 正文语种 eng
  • 中图分类 生物化学;
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