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首页> 外文期刊>Molecular biology of the cell >Myosin II-interacting guanine nucleotide exchange factor promotes bleb retraction via stimulating cortex reassembly at the bleb membrane
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Myosin II-interacting guanine nucleotide exchange factor promotes bleb retraction via stimulating cortex reassembly at the bleb membrane

机译：肌蛋白II-相互作用的鸟嘌呤核苷酸交换因子通过刺激皮质重组在BLEB膜上促进BLEB缩回

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摘要

Blebs are involved in various biological processes such as cell migration, cytokinesis, and apoptosis. While the expansion of blebs is largely an intracellular pressure-driven process, the retraction of blebs is believed to be driven by RhoA activation that leads to the reassembly of the actomyosin cortex at the bleb membrane. However, it is still poorly understood how RhoA is activated at the bleb membrane. Here, we provide evidence demonstrating that myosin II-interacting guanine nucleotide exchange factor (MYOGEF) is implicated in bleb retraction via stimulating RhoA activation and the reassembly of an actomyosin network at the bleb membrane during bleb retraction. Interaction of MYOGEF with ezrin, a well-known regulator of bleb retraction, is required for MYOGEF localization to retracting blebs. Notably, knockout of MYOGEF or ezrin not only disrupts RhoA activation at the bleb membrane, but also interferes with nonmuscle myosin II localization and activation, as well as actin polymerization in retracting blebs. Importantly, MYOGEF knockout slows down bleb retraction. We propose that ezrin interacts with MYOGEF and recruits it to retracting blebs, where MYOGEF activates RhoA and promotes the reassembly of the cortical actomyosin network at the bleb membrane, thus contributing to the regulation of bleb retraction.
机译：血液涉及各种生物过程，如细胞迁移，细胞因子和凋亡。虽然填充的膨胀在很大程度上是细胞内的压力驱动的过程，但据信抑制的缩回是由rhOA活化驱动的，这导致肌瘤膜皮层的重新组装在BLEB膜上。然而，它仍然令人难度的理解如何在BLEB膜上激活RHOA。在这里，我们提供了证明肌球蛋白II-相互作用的鸟嘌呤核苷酸交换因子（Myogef）通过刺激RhOA活化和在BLEB膜在BLEB缩回期间的肌动素网络的重组中涉及BLEB缩回。 Myogef与Ezrin的相互作用，一个已知的BLEB撤退调节器，Myogef定位需要缩回到雷布布。值得注意的是，Myogef或Ezrin的敲除不仅破坏了BLEB膜的RHOA活化，而且还会干扰非气体肌蛋白II定位和活化，以及在缩回填充中的肌动蛋白聚合。重要的是，Myogef淘汰会减慢BLEB Retraction。我们建议ezrin与Myogef相互作用，并募集到缩回损伤，其中Myogef激活RhOA并促进皮质膜在BLEB膜上的重新组装，从而有助于对BLEB缩回的调节。

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