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首页> 外文期刊>Molecular Immunology >Apa2H1, the first head domain of Apa2 trimeric autotransporter adhesin, activates mouse bone marrow-derived dendritic cells and immunization with Apa2H1 protects against Actinobacillus pleuropneumoniae infection
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Apa2H1, the first head domain of Apa2 trimeric autotransporter adhesin, activates mouse bone marrow-derived dendritic cells and immunization with Apa2H1 protects against Actinobacillus pleuropneumoniae infection

机译：APA2H1，APA2三聚体自耦运动员粘附粘蛋白的第一头域，用APA2H1激活小鼠骨髓衍生的树突细胞，免疫免疫保护Actinobacillus pleuropneumoniae感染

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Actinobacillus pleuropneumoniae is the causative pathogen of porcine pleuropneumonia, which results in large economic losses in the pig industry worldwide. There are, however, no effective subunit vaccines are available in the market owing to the various serotypes and the absence of cross-protection against this pathogen. Therefore, the selection of protective components is of great significance for vaccine development. We previously showed that trimeric autotransporter adhesins are important virulence factors of A. pleuropneumoniae. To determine the potential role in vaccine development of the functional head domain (Apa2H1) of Apa2, a trimeric autotransporter adhesin found in A. pleuropneumoniae, we obtained nature-like trimeric Apa2H1 using a prokaryotic expression system and co-culture of Apa2H1 with bone marrow derived dendritic cells (BMDCs) in vitro resulted in maturation-of BMDCs, characterised by the up-regulation of CD83, MHC-II, CCR7, ICAM-I and the increased expression of factors related to B lymphoid cells stimulation, such as proliferation-inducing ligand (APRIL), B lymphocyte stimulator (BLyS) and B cell activating factor (BAFF). The in vivo results showed that vaccination with Apa2H1 resulted in the robust production of antigen-specific antibodies, modestly induced mixed Th1 and Th2 immunity, impaired bacterial colonization and dissemination, and improved mouse survival rates. This study is the first to show that Apa2H1 is antigenic and can be used as a component of a subunit vaccine against A. pleuropneumoniae infection, providing valuable reference material for the development of an effective vaccine against A. pleuropneumoniae. (C) 2016 Elsevier Ltd. All rights reserved.
机译：Actinobacillus Pleuropneumoniae是猪胸膜炎的致病病原体，这导致全球猪工业的经济损失很大。然而，由于各种血清型和对该病原体的不存在交叉保护，市场上没有有效的亚基疫苗。因此，保护​​组分的选择对于疫苗发育具有重要意义。我们以前表明三聚体自耦体粘附素是A.Pleuropneumonee的重要毒力因子。为了确定APA2的功能头部结构疫苗（APA2H1）的疫苗开发中的潜在作用，在A.Pleuropneumoniae中发现的三聚体自耦体粘蛋白，我们使用骨髓的原核表达系统和APA2H1的共培养，获得了性质样的三聚体APA2H1在体外衍生的树突细胞（BMDC）导致BMDC的成熟，其特征在于CD83，MHC-II，CCR7，ICAM-I的上调和与B淋巴细胞刺激相关的因素的表达，例如增殖 - 诱导配体（4月），B淋巴细胞刺激剂（BLYS）和B细胞活化因子（BAFF）。体内结果表明，具有APA2H1的疫苗接种导致抗原特异性抗体的鲁棒生产，适度诱导的混合Th1和Th2抗扰度，受损的细菌定植和传播，以及改善的小鼠存活率。本研究是第一个显示APA2H1是抗原性的，可以用作亚基疫苗的组分，其针对A.胸膜肺炎感染，为对A.Pleuropneumonee的有效疫苗的开发提供有价值的参考材料。 （c）2016 Elsevier Ltd.保留所有权利。

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