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首页> 外文期刊>Crystallography reports >Implementation of Interference in a Confocal Laser Scanning Microscope and Its Application for In Situ Observation of Crystal Growth Processes
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Implementation of Interference in a Confocal Laser Scanning Microscope and Its Application for In Situ Observation of Crystal Growth Processes

机译:共焦激光扫描显微镜中干涉的实现及其在晶体生长过程的原位观察中的应用

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摘要

It is shown that interference can be implemented in the optical scheme of an Olimpus Lext-3000 laser scanning microscope. The occurring interference patterns are differently localized in space, depending on the experimental conditions. Interference from opposite crystal (volume) faces can be observed under certain conditions, which makes it possible to visualize (with a high sensitivity) defects that are invisible in polarized light and the surface relief. Misorientations and deformations in a crystal can be estimated. Our analysis allowed us to interpret the in situ observed interference pattern from growing lisozyme protein crystals and K2Co(SO4)(2) center dot 6H(2)O crystals.
机译:结果表明,可以在Olimpus Lext-3000激光扫描显微镜的光学方案中实现干涉。取决于实验条件,发生的干扰模式在空间上的定位不同。在某些条件下,可以观察到相对的晶体(体积)面的干涉,这使得可视化(高灵敏度)在偏振光和表面起伏中不可见的缺陷成为可能。可以估计晶体中的错误取向和变形。我们的分析使我们能够解释从生长的同工酶蛋白晶体和K2Co(SO4)(2)中心点6H(2)O晶体的原位观察到的干扰模式。

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