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首页> 外文期刊>Molecular phylogenetics and evolution >Untangling a mess of worms: Species delimitations reveal morphological crypsis and variability in Southeast Asian semi-aquatic earthworms (Almidae, Glyphidrilus)
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Untangling a mess of worms: Species delimitations reveal morphological crypsis and variability in Southeast Asian semi-aquatic earthworms (Almidae, Glyphidrilus)

机译：一个乱七八糟的蠕虫：物种分隔揭示了东南亚半水生蚯蚓（Almidae，Glyphidrilus）的形态克里普斯和可变性

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摘要

Semi-aquatic freshwater earthworms in the genus Glyphidrilus from Southeast Asia are characterized by both an extreme morphological crypsis among divergent phylogenetic lineages and a high morphological variability within the same phylogenetic lineages. The present study provides a new taxonomic framework for this problematic genus in SE Asia by integrating DNA sequence and morphological data. When single-locus and multilocus multispecies coalescent-based (MSC) species delimitation methods were applied to DNA sequence data, they usually yielded highly incongruent results compared to morphology-based species identifications. This suggested the presence of several cryptic species and high levels of intraspecific morphological variation. Applying reciprocal monophyly to the cytochrome c oxidase subunit 1 (COI) gene tree allowed us to propose the existence of 33 monophyletic species. Yet, often substantially more molecular operational taxonomic units (MOTUs) were obtained when species delimitation was based on COI and 16S rRNA sequences. In contrast, the ITS1 and ITS2 sequences suggested fewer MOTUs and did not recover most of the monophyletic species from the Mekong basin. However, several of these latter taxa were better supported when MSC species delimitation methods were applied to the combined mtDNA and ITS datasets. The ITS2 secondary structure retrieved one unnamed Mekong basin species that was not uncovered by the other methods when applied to ITS2 sequences. In conclusion, based on an integrative taxonomic workflow, 26 Glyphidrilus candidate species were retained and two remained to be confirmed. As such, this study provides evidence to suggest nine species new to science and to synonymize 12 nominal morphospecies. It also illustrates that the uncritical use of COI as a universal DNA barcode may overestimate species diversity because COI may be unable to distinguish between divergent conspecific lineages and different candidate species.
机译：来自东南亚的血小杂性蚯蚓中的半水生淡水蚯蚓，其特征在于不同的系统发育谱系中的极端形态胞嘧啶和同一系统发育谱系内的高形态变异性。本研究通过整合DNA序列和形态数据为SE亚洲的这种有问题的属性提供了新的分类框架。当单轨卡和多层多数聚合的基于聚合的（MSC）物种划分方法应用于DNA序列数据时，与基于形态的物种鉴别相比，它们通常会产生高度的不一致结果。这表明存在几种神秘种类和高水平的拆放形态变异。将往复式施用到细胞色素C氧化酶亚基1（COI）基因树允许我们提出33种单细胞物种的存在。然而，当物种界定基于COI和16S rRNA序列时，获得了通常更多的分子运作分类单位（MOTUS）。相比之下，ITS1和ITS2序列表明了较少的Motus，并没有从湄公河流域恢复大部分单晶物种。然而，当将MSC物种划定方法应用于组合的MTDNA及其数据集时，这些后者的几个分类群中的几种。 ITS2二级结构检索了在施加到其2序列时未被其他方法未发现的一个未命名的湄公河盆地物种。总之，基于综合分类工作流程，保留了26种格利氏菌候选物种，保留了两种候选物种。因此，本研究提供了表明九种科学新物种，并同义了12个名义的形态学。它还示出了COI作为通用DNA条形码的非临界使用可能超过物种多样性，因为COI可能无法区分不同的内特异性谱系和不同的候选物种。

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