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首页> 外文期刊>Mycorrhiza >Root colonization and arbuscular mycorrhizal fungal community composition in a genetically modified maize, its non-modified isoline, and a landrace
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Root colonization and arbuscular mycorrhizal fungal community composition in a genetically modified maize, its non-modified isoline, and a landrace

机译:在遗传修饰的玉米,其未经修改的isoline和兰德斯和地兰的根殖殖民化和丛枝菌根真菌群落组成

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摘要

The use of genetically modified (GM) plants has increased in recent decades, but there are uncertainties about their effects on soil microbial communities. Aiming to quantify root colonization and characterize arbuscular mycorrhizal fungi (AMF) communities associated with roots and rhizosphere soil of different maize genotypes, a field trial was carried out in Southern Brazil with three maize genotypes as follows: a GM hybrid (DKB 240 VTPRO), its non-modified isoline (DKB 240), and a landrace (Pixurum). Soil samples were collected to evaluate the occurrence of AMF during the growth of corn genotypes at sowing and V3 (vegetative), R1 (flowering), and R3 (grain formation) stages of the crop. The occurrence of AMF was determined by the morphological identification of spores, and by analyzing AMF community composition in soil and roots of maize, using PCR-DGGE. The GM genotype of maize promoted lower mycorrhizal colonization in the vegetative stage and had lower sporulation at grain development than the conventional hybrid and the landrace maize. Twenty AMF morphotypes were identified and 13 were associated with all maize genotypes. The generaAcaulospora,Glomus, andDentiscutatahad the largest numbers of species. There were no differences in AMF community composition due to maize genotypes or genetic modification, but crop phenological stages affected AMF communities associated with maize roots.
机译:近几十年来,使用遗传改性(GM)植物的使用,但它们对土壤微生物社区的影响存在不确定性。旨在量化具有不同玉米基因型的根和根际土壤与不同玉米基因型相关的丛核化性和表征丛核菌根(AMF)群落,在巴西南部进行了田间试验,如下三种玉米基因型:GM杂交(DKB 240 VTPRO),其未修饰的ISOLALE(DKB 240)和LANDRACE(PIXURUM)。收集土壤样品以评估播种和v3(植物),R1(开花)和作物的R3(籽粒形成)阶段的玉米基因型生长期间的AMF的发生。通过孢子的形态鉴定,通过PCR-DGGE分析玉米土壤和根部的AMF群落组合物,确定AMF的发生。玉米的GM基因型促进了植物阶段的菌根腐殖化降低,谷物开发的孢子率低于传统的杂交和兰德玉米。鉴定了二十AMF Mor型型,13个与所有玉米基因型相关。 Glyaauraulospora,Glomus,Anddentiscutatahad是最多的物种。由于玉米基因型或遗传修饰,AMF群落组成没有差异,但农作物鉴定与玉米根相关的AMF社区影响。

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  • 来源
    《Mycorrhiza》 |2020年第5期|共11页
  • 作者单位

    Univ Fed Santa Catarina Ctr Ciencias Agr Dept Engn Rural Rodovia Admar Gonzaga 1346 BR-88034001 Florianopolis SC Brazil;

    Univ Fed Santa Catarina Ctr Ciencias Agr Dept Engn Rural Rodovia Admar Gonzaga 1346 BR-88034001 Florianopolis SC Brazil;

    Ctr Univ Catolica Santa Catarina Joinville Campus Joinville Rua Visconde de Taunay 427 BR-89203005 Joinville SC Brazil;

    Univ Fed Santa Catarina Ctr Ciencias Biol Dept Microbiol Imunol &

    Parasitol Campus Reitor Joao David Ferreira Lima BR-88040900 Florianopolis SC Brazil;

    Ctr Univ Catolica Santa Catarina Joinville Campus Joinville Rua Visconde de Taunay 427 BR-89203005 Joinville SC Brazil;

    Univ Fed Santa Catarina Ctr Ciencias Biol Dept Ecol &

    Zool Campus Reitor Joao David Ferreira Lima BR-88040900 Florianopolis SC Brazil;

    Fundacao Univ Reg Blumenau Ctr Ciencias Exatas &

    Nat Dept Ciencias Nat Rua Antonio da Veiga 140 BR-89030903 Blumenau SC Brazil;

    Univ Fed Santa Catarina Ctr Ciencias Agr Dept Fitotecnia Rodovia Admar Gonzaga 1346 Bloco B BR-88040900 Florianopolis SC Brazil;

    Univ Fed Santa Catarina Ctr Ciencias Biol Dept Microbiol Imunol &

    Parasitol Campus Reitor Joao David Ferreira Lima BR-88040900 Florianopolis SC Brazil;

    Univ Fed Santa Catarina Ctr Ciencias Agr Dept Engn Rural Rodovia Admar Gonzaga 1346 BR-88034001 Florianopolis SC Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 真菌门;
  • 关键词

    AMF; Corn; GM crops; PCR-DGGE; Phenological stages; Zea maysL;

    机译:amf;玉米;gm作物;pcr-dgge;毒性阶段;zea maysl;

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