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首页> 外文期刊>Mycorrhiza >Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants
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Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants

机译：Terfezia Claveryi的菌丝体作为孤园源生产沙漠块状菌根菌根植物

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摘要

Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Bchnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L-1 dry weight and productivity increased from 10.7 to 95.8 mg L(-1 )day(-1) dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.
机译：Terfezia Claveryi Chatin是第一个栽培的沙漠松露物种，菌根植物通过使用沙漠松露孢子和菌丝体成功生产。然而，尽可能使用比孢子和有利可图的菌丝体。鉴于使用这种松露的传统培养方法获得的菌丝菌菌丝的低产量，修饰了培养基组成，试图确定其营养要求。为此，使用BOX-BCHNKEN设计进行涉及响应面方法的测定，以找到高生产菌丝体生物质的最佳参数。用葡萄糖作为碳源获得最佳结果，在培养期间缓冲pH，加入维生素，并调节MMN培养基的最佳浓度的碳和氮源。生物质产量从0.3〜3g L-1干重增加，生产率从10.7增加到95.8 mg L（-1）天（-1）干重。生产的菌丝体能够有效地殖民殖民地根源，提供超过50％的突出菌腐殖殖民化。

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