• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular Breeding >Development and application of indica-japonica SNP assays using the Fluidigm platform for rice genetic analysis and molecular breeding
【24h】

Development and application of indica-japonica SNP assays using the Fluidigm platform for rice genetic analysis and molecular breeding

机译：用水稻遗传分析和分子育种流体平台的籼粳SNP测定的开发与应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molecular markers are efficient and essential genotyping tools for molecular breeding and genetic analysis of rice. We developed two 96-plex indica-japonica single nucleotide polymorphism (SNP) genotyping sets for genetic analysis and molecular breeding in rice using the Fluidigm platform. Informative SNPs between indica and japonica were selected from SNP data of the Rice Diversity database, HapRice world SNP data of the Q-TARO database, and our 40 rice cultivar resequencing dataset. SNPs in set 1 were evenly distributed across all 12 rice chromosomes at a spacing of 4-5 Mb between adjacent SNPs. SNPs in set 2 mapped to the long genetic intervals in set 1 and included 14 functional or linked SNPs in genes previously cloned and associated with agronomic traits. Additionally, we used the SNP sets developed in this study to perform genetic diversity analysis of various cultivated and wild rice accessions, construction and validation of a subspecies diagnostic subset, linkage map construction and quantitative trait locus (QTL) analysis of a japonica x indica F-2 population, and background profiling during marker-assisted backcrossing. Furthermore, we identified subspecies-specific SNPs and discuss their distribution and association with agronomic traits and subspecies differentiation. Our results indicate that these subspecies-specific SNPs were present in wild rice prior to domestication. This genotyping system will serve as an efficient and quick tool for genetic analysis and molecular breeding in rice.
机译：分子标记是水稻分子育种和遗传分析的有效且基本的基因分型工具。我们使用液晶平台开发了两种96-吡格蛋白粳稻单核苷酸多态性（SNP）基因分型，用于稻米遗传分析和分子育种。 Indega和Japonic之间之间的信息SNP选自米多样性数据库的SNP数据，Q-Taro数据库的Haprice世界SNP数据，以及我们的40个稻米品种Resequecing数据集。 SEN中的SNP在相邻的SNP之间的间距为4-5 MB的所有12米染色体上均匀分布。 SEN 2中的SNP映射到SET 1中的长遗传间隔，并包含在以前克隆的基因中的14个功能或连接的SNP，并与农艺性状相关。此外，我们使用本研究中开发的SNP集合，以对各种栽培和野生水稻的遗传多样性分析，亚种诊断子集的建设和验证，联系地图构建和定量特质基因座（QTL）分析的粳稻x indica f -2人口，以及在标记辅助回复期间的背景分析。此外，我们确定了特定于亚种的SNP，并与农艺性状和亚种分化讨论其分发和关联。我们的结果表明，在驯化之前，这些亚种特异性SNP存在于野生稻中。该基因分型系统将作为水稻遗传分析和分子育种的有效和快速的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号