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首页> 外文期刊>Mycobiology >Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea
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Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea

机译：原生质体制备和药用真菌antrodia cinnamomea的优化

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摘要

Antrodia cinnamomea is a unique medicinal fungus in Taiwan. It has been found rich in some pharmacologically active compounds for anti-cancer, hangover, and immune regulation etc. With the in-depth study of these components, it would be interesting and important to establish a molecular system for basic studies of A. cinnamomea. Thus, we would like to set up a foundation for this purpose by studying the A. cinnamomea protoplast preparation and regeneration. Firstly, we studied the optimization method of protoplast preparation of A. cinnamomea, and found various factors that may affect the yield during protoplast preparation, such as mycelial ages, pH values, and osmotic stabilizers. Secondly, in the regeneration of protoplasts, we explored the effects of various conditions on the regeneration of protoplasts, including different media and osmotic pressure. In addition, we found that citrate buffer with pH value around 3 dramatically increased the regeneration of protoplasts of A. cinnamomea, and provided a set of regeneration methodology for A. cinnamomea.
机译：Antrodia Cinnamomea是台湾独特的药用真菌。它已被发现富含一些药理学活性化合物用于抗癌，宿醉和免疫调节等。利用这些组分的深入研究，建立A.肉桂瘤的基本研究的分子系统是有趣的，很重要。因此，我们希望通过研究A.肉桂膜原生质体制备和再生来为此目的进行基础。首先，我们研究了A.肉桂瘤的原生质体制备的优化方法，发现了各种可能影响原生质体制剂中产量的各种因素，例如菌丝体，pH值和渗透稳定剂。其次，在原生质体的再生中，我们探讨了各种条件对原生质体再生的影响，包括不同培养基和渗透压。此外，我们发现具有pH值的柠檬酸盐缓冲液大大显着增加了A.肉桂瘤的原生质体的再生，并为肉桂瘤提供了一组再生方法。

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