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Phytophthora acaciae sp. nov., a new species causing gummosis of black wattle in Brazil

机译:Phytophthora acaciae sp。 11月。,一种造成巴西黑色荆棘肠道的新物种

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摘要

A new Phytophthora species was found associated with gummosis in black wattle plantations in the subtropical, humid, south of Brazil. The new species Phytophthora acaciae is formally named herein based on phylogenetic and morphological analyses. This is the fourth Phytophthora species found from this pathogen complex in black wattle plantations causing gummosis in Brazil. The other three species are P. nicotianae, P. boehmeriae, and P. frigida. Phytophthora acaciae is heterothallic with amphigynous antheridia, noncaducous, papillate sporangia and is placed in the Phytophthora clade 2 based on nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) sequences. Maximum parsimony and maximum likelihood phylogenetic analyses of P. acaciae isolates based on multigene sequences, including partial DNA sequences of three nuclear protein-coding genes (beta-tubulin, translation elongation factor-1 alpha, and ras-related protein), two mitochondrial protein-coding genes (cytochrome c oxidase subunits I and II), in addition to ITS sequence data, support the delimitation of this new species on Acacia mearnsii from the other previously described clade 2 Phytophthora species. Pathogenicity trial confirmed that the new species causes necrotic lesions on the plant stem, with either the presence or absence of gum.
机译:发现了一种新的植物,在巴西南部的亚热带,潮湿的黑粉种植园中的胶质瘤相关。新物种植物植物植物胰岛素基于系统发育和形态学分析在本文中正式命名。这是从这种病原体复合物中发现的第四种植物学,在巴西引起胶囊的胶囊。另外三种物种是p. nicotianae,p. boehmeriae和p. frigida。 Phytophthora Acaciae是杂种属,具有阳性痰湿,非粘性乳头孢子囊,并基于NUC RDNA内转录的间隔物(其1-5.8S-ITS2 = ITS)序列置于植物滴黄素2中。基于多聚烯序列的P.Acaciae分离物的最大判例和最大似然性系统发育分析,包括三个核蛋白编码基因的部分DNA序列(β-小管蛋白,翻译延伸因子-1α和RAS相关蛋白),两个线粒体蛋白 - 除了其序列数据之外,还支持基因(细胞色素C氧化酶I和II),支持从另一个先前描述的植物学2种植物藻苜蓿物种上对血清生效的这种新物种的限制。致病性试验证实,新物种导致植物茎上的坏死病变,具有牙龈的存在或不存在。

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