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首页> 外文期刊>Mycologia >Multilocus phylogeny of the Trichophyton mentagrophytes species complex and the application of matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry for the rapid identification of dermatophytes
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Multilocus phylogeny of the Trichophyton mentagrophytes species complex and the application of matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry for the rapid identification of dermatophytes

机译：滴毛术术术术复合物的多层植物发育及基质辅助激光解吸/电离 - 飞行时间（MALDI-TOF）质谱法的快速鉴定皮肤病

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Despite intensive studies of the Trichophyton mentagrophytes species complex, its taxonomy still causes confusion. In this study, more than 70 dermatophytes were analyzed based on nuc rDNA ITS1-5.8S-ITS2 (ITS), D1-D2 domains of nuc 28S rDNA (D1D2), and beta-tubulin gene (TUBB) sequences to clarify phylogenetic relationships in the complex. This demonstrated that strains of the complex were divided into three major lineages with high statistical support: (i) T. benhamiae and related species; (ii) T. simii and two related species, T. quinckeanum and T. schoenleinii; and (iii) T. mentagrophytes, T. interdigitale, and related species. The major lineages could be further divided into 18 phylogroups, representing either individual species or phylogenetically distinct groups within species. Among strains of T. benhamiae, African isolates American Type Culture Collection (ATCC) 28064 and 28065 formed a phylogenetically distinct phylogroup from their type strain and were considered a distinct species. Strains of T. mentagrophytes were divided into at least four phylogroups based on combined sequence analysis, but some phylogroups showed closer relationships to T. interdigitale, T. equinum, and T. tonsurans when compared by individual genes. This indicates that identifying those species with one gene could lead to incorrect results. For rapid identification of those dermatophytes, each phylogroup was tested by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry using a database with customized reference spectra of each phylogroup. This system was able to identify all the tested strains to species level with higher than 91% accuracy, except for strains of T. interdigitale. The three phylogroups of T. benhamiae were well distinguished from one another with high identification accuracy, whereas phylogroups of T. mentagrophytes were often cross-identified to one another or to T. interdigitale. Further research should improve identification accuracy for some species, but the results suggested that MALDI-TOF MS could be a rapid and efficient identification tool for closely related dermatophytes in the T. mentagrophytes species complex.
机译：尽管对滴虫术术术群体复杂的密集研究，但其分类物仍然会引起混淆。在该研究中，基于NUC RDNA的NUC RDNA ita1-5.8S-ITS2（ITS），NUC 28S RDNA（D1D2）的D1-D2结构域，以及β-小管蛋白基因（TUBB）序列的β-微管蛋白基因（TUBB）序列进行分析超过70种皮肤细胞。复杂。这证明了复合物的菌株分为三个主要谱系，具有高统计学支持：（i）T.Benhamiae和相关物种; （ii）T.Imii和两个相关的物种，T. Quinckeanum和T. Schoenleinii; （III）T.宫颈癣，T. Interditale和相关物种。主要谱系可以进一步分为18个植物粒，代表物种内的个体种类或系统源性不同的群体。在T.Benhamiae的菌株中，非洲分离美国型培养物收集（ATCC）28064和28065从其型菌株中形成了系统源性不同的文学组，并且被认为是一种不同的物种。基于组合的序列分析，将T.术的菌株分成至少四个植物群中，但是通过个体基因比较，一些植物组显示与T. Interdigitale，T.Entditale，T.Enthinum和T. uncorans的关系更接近。这表明鉴定具有一个基因的这些物种可能导致结果不正确。为了快速鉴定那些皮肤病，通过基质辅助激光解吸/电离 - 飞行时间（MALDI-TOF）质谱法试验每个晶粒组，所述数据库使用具有每种晶粒的定制参考光谱的数据库。除了T. Interditale的菌株之外，该系统能够将所有测试菌株识别到物种水平，以高于91％的精度。 T.Benhamiae的三个晶粒很好地与彼此的识别精度彼此区分开，而T.术后的术术常将彼此交叉鉴定或interdigitale。进一步的研究应该改善某些物种的鉴定准确性，但结果表明MALDI-TOF MS可以是一种快速有效的识别工具，用于T.Mentagrophytes物种复合物中的密切相关的皮肤病。

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