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首页> 外文期刊>Mycologia >Identification and characterization of Septoria steviae as the causal agent of Septoria leaf spot disease of stevia in North Carolina
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Identification and characterization of Septoria steviae as the causal agent of Septoria leaf spot disease of stevia in North Carolina

机译：Sememoria Steviae作为北卡罗来纳州甜叶菊Sememoria叶斑病的因果剂的鉴定与表征

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摘要

Stevia (Stevia rebaudiana) is an emerging perennial crop in the southeastern United States. A Septoria leaf spot disease of stevia was first identified on field plantings in Japan in 1978. The pathogen was named Septoria steviae based on a morphological characterization. In 2015, a species of Septoria with morphological characters of S. steviae was isolated from field and greenhouse-grown stevia plants with leaf spot symptoms in North Carolina. In this study, 12 isolates obtained from diseased stevia plants in 2015 and 2016 were characterized and compared with reference strains of S. steviae. Comparisons were based on conidial and pycnidial morphology and multilocus sequence analysis of actin (ACT), beta-tubulin (BT), calmodulin (CAL), nuc rDNA internal transcribed spacers (ITS1-5.8S-ITS2 = ITS), nuc rDNA 28S subunit (28S), RNA polymerase II second largest subunit (RPB2), and translation elongation factor-1 alpha (TEF1). Measurements of conidia and pycnidia from symptomatic field leaves and 12 pure cultures grown on nutrient medium were consistent with those previously reported for ex-type strains of S. steviae. North Carolina strains formed a well-supported monophyletic group with ex-type strains of S. steviae. This study represents the first genetic characterization of S. steviae in the United States and provides an experimental framework to elucidate the genetic diversity and disease ecology of field populations of S. steviae.
机译：甜叶虫（甜叶菊莱巴伯邦）是美国东南部的新兴多年生植物作物。 1978年首先在日本的田间种植中首先确定了甜叶菊的静脉叶斑病。该病原体基于形态表征被命名为SemoRia Steviae。 2015年，一家儿女群体的血管生物形态特征与北卡罗来纳州的叶斑病症状的田间和温室生长的甜叶菊植物中分离出来。在这项研究中，将于2015年和2016年从患病甜叶菊植物获得的12分离株，并与S.Steviae的参考菌株进行了表征和比较。比较基于分析和肌动蛋白（ACT），β-微管蛋白（BT），钙调蛋白（CAL），NUC RDNA内转录的间隔物（ITS1-5.8S-ITS2 = ITS），NUC RDNA 28S亚基的分析和Pycnidial序列分析（28s），RNA聚合酶II第二大亚基（RPB2）和翻译延伸因子-1α（TEF1）。从症状野外叶片和12种营养培养基生长的纯培养物的测量结果与先前报道的S.Steviae的Ex型菌株的含量一致。北卡罗来纳菌株形成了一种良好的支持良好的单级单位群，具有甜叶氏菌菌株。该研究代表了美国S. Steviae的第一个遗传表征，并提供了一个实验框架，以阐明S. Steviae的野外群体的遗传多样性和疾病生态学。

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