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首页> 外文期刊>Molecular reproduction and development >Quisinostat treatment improves histone acetylation and developmental competence of porcine somatic cell nuclear transfer embryos
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Quisinostat treatment improves histone acetylation and developmental competence of porcine somatic cell nuclear transfer embryos

机译：Quisinostat治疗改善了猪体细胞核转移胚的组蛋白乙酰化和发育能力

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摘要

Abnormal epigenetic modifications are considered a main contributing factor to low cloning efficiency. In the present study, we explored the effects of quisinostat, a novel histone deacetylase inhibitor, on blastocyst formation rate in porcine somatic-cell nuclear transfer (SCNT) embryos, on acetylation of histone H3 lysine 9 (AcH3K9), and on expression of POU5F1 protein and apoptosis-related genes BAX and BCL2. Our results showed that treatment with 10 nM quisinostat for 24 hr significantly improved the development of reconstructed embryos compared to the untreated group (19.0 +/- 1.6% vs. 10.2 +/- 0.9%; p 0.05). Quisinostat-treated SCNT embryos also possessed significantly increased AcH3K9 at the pseudo-pronuclear stage (p 0.05), as well as improved immunostaining intensity for POU5F1 at the blastocyst stage (p 0.05). While no statistical difference in BAX expression was observed, BCL2 transcript abundance was significantly different in the quisinostat-treated compared to the untreated control group. Of the 457 quisinostat-treated cloned embryos transferred into three surrogates, six fetuses developed from the one sow that became pregnant. These findings suggested that quisinostat can regulate gene expression and epigenetic modification, facilitating nuclear reprogramming, and subsequently improving the developmental competence of pig SCNT embryos and blastocyst quality.
机译：异常表观遗传修饰被认为是低克隆效率的主要贡献因素。在本研究中，我们探讨了Quisinostat，一种新型组蛋白脱乙酰酶抑制剂对猪体细胞核转移（SCNT）胚胎胚囊形成速率的影响，对组蛋白H3赖氨酸9（ACH3K9）的乙酰化，以及POU5F1的表达蛋白质和凋亡相关基因Bax和Bcl2。我们的研究结果表明，与未处理基团相比，用10nm Quisinostat治疗24小时的Quisinostat 24小时的开发重建胚胎（19.0 +/- 1.6％与10.2 +/- 0.9％; p <0.05）。 Quisinostat治疗的SCNT胚胎在伪核阶段（P <0.05）处也具有显着增加的ACH3K9，以及在胚泡阶段（P <0.05）处的POU5F1的免疫染色强度。虽然观察到BAX表达的统计差异，但与未处理对照组相比，BCL2转录物在Quisinostat治疗中有显着差异。将457个Quisinostat治疗的克隆胚胎转移到三种替代物中，从怀孕的一个母猪中产生了六个胎儿。这些研究结果表明，Quisinostat可以调节基因表达和表观遗传修饰，促进核重编程，随后提高猪Scnt胚胎和胚泡质量的发育能力。

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