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首页> 外文期刊>CrystEngComm >Controllable synthesis of amine-functionalized Fe3O4 polyhedra for lipase immobilization
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Controllable synthesis of amine-functionalized Fe3O4 polyhedra for lipase immobilization

机译：固定化脂肪酶的胺官能化Fe3O4多面体的可控制合成

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摘要

We developed an efficient strategy to synthesize Fe3O4 truncated cubes, rhombic dodecahedra and octahedra with exposed {100}/{110}, {110} and {111} facets, respectively. Fourier transform infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS) spectra indicated that the Fe3O4 polyhedra were functionalized with a -NH2 group. Magnetic measurements revealed that the prepared products have a high saturation magnetization and low coercive force. Because of the magnetic nature and amine-functionalized surfaces, the as-synthesized Fe3O4 polyhedra were employed as ideal magnetic carriers for lipase immobilization. Bradford protein assays indicated that the lipase loading amounts on Fe3O4 truncated cubes, rhombic dodecahedra and octahedra were 174.7, 175.6 and 175.8 mg of protein per g, respectively. Thermal stability tests revealed that the lipase immobilized on Fe3O4 octahedra was bound with great efficiency preserving the high catalytic activity and amplifying the stability. Reusability assays indicated that the lipase immobilized on Fe3O4 octahedra can be reused 4 times without a marked loss of catalytic activity (<8%). This work demonstrated that the prepared Fe3O4 octahedra were more stable and had promising applications in biological fields.
机译：我们开发了一种有效的策略来合成Fe3O4截短的立方体，菱形十二面体和八面体，分别暴露了{100} / {110}，{110}和{111}面。傅立叶变换红外（FT-IR）和X射线光电子能谱（XPS）光谱表明，Fe3O4多面体被-NH2基团官能化。磁性测量表明所制备的产品具有高饱和磁化强度和低矫顽力。由于磁性和胺官能化的表面，已合成的Fe3O4多面体被用作固定脂肪酶的理想磁性载体。布拉德福德蛋白质测定法表明，每克Fe3O4截短的立方体，菱形十二面体和八面体上的脂肪酶负载量分别为174.7、175.6和175.8 mg蛋白/ g。热稳定性测试表明，固定在Fe3O4八面体上的脂肪酶具有很高的结合效率，可以保持较高的催化活性并增强稳定性。可重用性分析表明，固定在Fe3O4八面体上的脂肪酶可重复使用4次，而催化活性没有明显损失（<8％）。这项工作表明，制备的Fe3O4八面体更稳定，在生物领域具有广阔的应用前景。

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