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首页> 外文期刊>Nanotoxicology >Nanoparticle size-specific actin rearrangement and barrier dysfunction of endothelial cells
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Nanoparticle size-specific actin rearrangement and barrier dysfunction of endothelial cells

机译：纳米粒子尺寸特异性肌动蛋白重排和内皮细胞的阻隔功能障碍

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In this work, we evaluated the impact of gold nanoparticles on endothelial cell behavior and function beyond the influence on cell viability. Five types of gold nanoparticles were studied: 5nm and 20nm bare gold nanoparticles, 5nm and 20nm gold nanoparticles with biocompatible polyethylene glycol (PEG) coating and 60nm bare gold nanoparticles. We found that all tested gold nanoparticles did not affect cell viability significantly and reduced the reactive oxygen species (ROS) level in endothelial cells. Only 20nm bare gold nanoparticles caused an over 50% increase in endothelial barrier permeability and slow recovery of barrier function was observed after the gold nanoparticles were removed. This impairment in endothelial barrier function was caused by unbalanced forces between intracellular tensions and paracellular forces, actin microfilament rearrangement, which occurred through a Rho/ROCK kinase-dependent pathway and broke the force balance between intracellular tensions and paracellular forces. The size-specific effect of gold nanoparticles on endothelial cells may have important implications regarding the behavior of nanoparticles in the biological system and provide valuable guidance in nanomaterial design and biomedical applications.
机译：在这项工作中，我们评估了金纳米粒子对内皮细胞行为的影响，超出了细胞活力的影响。研究了五种类型的金纳米颗粒：5nm和20nm裸金纳米颗粒，5nm和20nm金纳米颗粒，具有生物相容性聚乙二醇（PEG）涂层和60nm裸金纳米颗粒。我们发现所有测试的金纳米颗粒都不会显着影响细胞活力并降低内皮细胞中的活性氧物质（ROS）水平。在除去金纳米颗粒后，仅引起20nm裸裸纳米颗粒引起内皮阻挡渗透性的增加超过50％的内皮阻挡渗透性增加，并观察到屏障功能的缓慢恢复。内皮屏障功能的这种损伤是由细胞内张力和荚膜压力之间的不平衡力引起的，肌动蛋白微丝重排通过rhO /岩石激酶依赖性途径发生，并在细胞内张力和柱细胞之间造成力平衡。金纳米粒子对内皮细胞的尺寸特异性效果可能对生物系统中纳米颗粒的行为具有重要意义，并提供纳米材料设计和生物医学应用中的有价值的指导。

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