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首页> 外文期刊>Nanotoxicology >An integrated approach to determine interactive genotoxic and global gene expression effects of multiwalled carbon nanotubes (MWCNTs) and benzo[a]pyrene (BaP) on marine mussels: evidence of reverse 'Trojan Horse' effects
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An integrated approach to determine interactive genotoxic and global gene expression effects of multiwalled carbon nanotubes (MWCNTs) and benzo[a]pyrene (BaP) on marine mussels: evidence of reverse 'Trojan Horse' effects

机译：一种综合方法，用于确定多壁碳纳米管（MWCNT）和苯并[a]芘（BAP）对海洋贻贝的互动遗传毒性和全局基因表达效应的综合方法：逆向'特洛伊木马'效果的证据

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摘要

The interactions between carbon-based engineered nanoparticles (ENPs) and organic pollutants might enhance the uptake of contaminants into biota. The present integrated study aimed to assess this potential 'Trojan Horse', probing the interactive effects of purpose-made multi-walled carbon nanotubes (MWCNTs), a representative ENP, and benzo[a]pyrene (BaP), a ubiquitous polycyclic aromatic hydrocarbon (PAH) pollutant, on the marine mussel Mytilus galloprovincialis. Mussels were exposed to MWCNTs and BaP either alone or in various combinations. The co-exposure of BaP with MWCNTs revealed that the presence of MWCNTs enhanced the aqueous concentrations of BaP, thereby reducing the uptake of this pollutant by mussels as evidenced by lowering BaP concentrations in the tissues. Determination of DNA damage (comet assay) showed a concentration-dependent response for BaP alone which was absent when MWCNTs were present. Global gene expression using microarray analyses indicated that BaP and MWCNTs, in combination, differentially activated those genes which are involved in DNA metabolism compared to the exposures of BaP or MWCNTs alone, and the gene expression response was tissue-specific. Mechanisms to explain these results are discussed and relate primarily to the adsorption of BaP on MWCNTs, mediated potentially by van der Waals interactions. The use of a novel approach based on gold-labeled MWCNTs to track their uptake in tissues improved the traceability of nanotubes in biological samples. Overall, our results did not indicate the 'Trojan Horse' effects following co-exposure to the contaminants and clearly showed that the adsorption of BaP to MWCNTs modified the uptake of the pollutant in marine mussels.
机译：碳基工程化纳米颗粒（ENP）和有机污染物之间的相互作用可能增强污染物的摄取到生物群中。目前的综合研究旨在评估该潜在的“特洛伊木马”，探测目的制造的多壁碳纳米管（MWCNT），代表性ENP和苯并[A]芘（BAP），一种普遍的多环芳烃烃的互动效果（PAH）污染物，在海洋贻贝肉豆蔻肠道病变。贻贝暴露于mwcnts，单独或以各种组合露出。 BAP与MWCNT的共曝光显示，MWCNT的存在增强了含水浓度的壳体，从而通过贻贝减少该污染物的摄取，这通过降低组织中的壳体浓度来证明。 DNA损伤的测定（COMET测定）显示出在存在MWCNTS时，单独的舒张效应依赖性响应。使用微阵列分析的全局基因表达表明，与单独的壳体或MWNTS的暴露相比，BAP和MWCNT组合，差异激活了参与DNA代谢的那些基因，并且基因表达响应是组织特异性。解释这些结果的机制主要讨论并涉及通过范德华相互作用介导的介导的BAP对MWCNT的吸附。使用基于金的MWCNT的新方法追踪其在组织中的摄取改善了纳米管在生物样品中的可追溯性。总体而言，我们的结果并未表明在共同暴露于污染物并清楚地表明对MWCNTS的吸附来调节污染物在海洋贻贝中的吸收。

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