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首页> 外文期刊>Natural product communications >Metabolomic and Proteomic Analysis of the Response of Angelica acutiloba after Herbivore Attack
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Metabolomic and Proteomic Analysis of the Response of Angelica acutiloba after Herbivore Attack

机译：食草动物攻击后当归acutiloba响应的代谢组和蛋白质组学分析

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To investigate the effect of insect damage on the biosynthesis of secondary metabolites, we cultivated Angelica acutiloba with and without damage caused by the larvae of Papilio machaon. Compounds from the leaves and roots of A. acutiloba were extracted with chloroform and analyzed by GC-MS. We annotated the peaks based on the mass spectral data and retention times. In addition, the effects of insect damage on the plants were investigated by principal component analysis (PCA). As a result, it was clarified that the amounts of ligustilide, gamma-terpinene and beta-caryophyllene, increased in leaves after being damaged by insects. Polyacetylenes also increased in the roots of damaged plants. In addition, as prompt responses, increases in the proteins relating to hydrogen peroxide synthesis and decreases in the proteins concerned with a non-urgent response to pathogenic attack were clarified by proteomic analysis. These results indicate that cultivation methods using the chemical-ecological response of the plant can contribute to the production of higher-quality crude drugs derived from A. acutiloba.
机译：为了探讨昆虫损伤对次生代谢物生物合成的影响，我们用Papilio Machaon幼虫造成的Angelica Aculiloba培养了Angelica Aculiloba。用氯仿萃取来自A. Acutiloba的叶子和根的化合物并通过GC-MS分析。我们基于质谱数据和保留时间向峰值注释。此外，通过主成分分析（PCA）研究了昆虫损伤对植物的影响。结果，澄清了昆虫破坏后叶片，γ-萜烯和β-羧基的量增加。损伤植物的根部也增加了聚乙炔。另外，通过蛋白质组学分析阐明了与过氧化氢合成有关的蛋白质的蛋白质增加和蛋白质中的蛋白质减少的蛋白质的增加。这些结果表明，使用该植物的化学生态反应的培养方法可以促进衍生自A型Aculiloba的高质量粗药物的生产。

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