...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanoscience and Nanotechnology Letters >microRNA-15b-5p Regulates Vascular Endothelial Growth Factor Signaling Through VEGFR2 in Human Umbilical Vein Endothelial Cells
【24h】

microRNA-15b-5p Regulates Vascular Endothelial Growth Factor Signaling Through VEGFR2 in Human Umbilical Vein Endothelial Cells

机译：MicroRNA-15B-5P通过VEGFR2调节人脐静脉内皮细胞VEGFR2的血管内皮生长因子信号传导

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

microRNAs (miRNAs) are post-transcriptional modulators of gene expression and play an important role in many processes of cardiovascular disease. Vascular endothelial cells can regulate signaling and material transmission between blood and tissue cells. These cells also participate in the process of sub-endothelial lipoprotein deposition and vascular inflammation. Here, we showed that miR-15b-5p could regulate VEGF signaling by targeting VEGFR2. Human umbilical vein endothelial cells (HUVECs) transfected with miR-15b-5p and its specific inhibitor along with luciferase report assay, qPCR, and western blotting were used to verify that miR-15b-5p targets VEGFR2. Overexpression of miR-15b-5p in HUVECs down-regulated VEGFR2 mRNA and protein expression and simultaneously down-regulated the expression of the downstream signaling molecule AKT. Furthermore, increased miR-15b-5p expression inhibited the proliferation of HUVECs and the expression of the anti-apoptosis gene BCL2 and induced the active pro-apoptosis gene caspase-3. Inhibiting the expression of miR-15b-5p by transfection with an miR-15b-5p inhibitor augmented the proliferation of the HUVECs. Transfection with miR-15b-5p suppressed migration and tube formation, which were attenuated by the miR-15b-5p inhibitor. miR-15b-5p down-regulated VEGF signaling via the AKT pathway; thus, the miR-15b-5p RNA inhibitor could be a possible therapeutic agent for cardiovascular disease.
机译：MicroRNAs（miRNA）是基因表达后转录调节剂，在许多心血管疾病过程中发挥着重要作用。血管内皮细胞可以调节血液和组织细胞之间的信号和材料透射。这些细胞还参与亚内皮脂蛋白沉积和血管炎症的过程。在这里，我们展示MIR-15B-5P可以通过靶向VEGFR2来调节VEGF信号传导。用MiR-15B-5P及其特异性抑制剂与荧光素酶报告测定，QPCR和Western印迹转染的人脐静脉内皮细胞（HUVEC）用于验证miR-15b-5p靶标VEGFR2。 Huvecs下调VEGFR2 mRNA和蛋白表达中MIR-15B-5P的过度表达，并同时下调下游信号分子AKT的表达。此外，增加的miR-15b-5p表达抑制Huvecs的增殖和抗凋亡基因Bcl2的表达，并诱导了活性促凋亡基因Caspase-3。通过用miR-15b-5p抑制剂转染抑制miR-15b-5p的表达增强了Huvecs的增殖。用miR-15b-5p抑制迁移和管形成转染，由miR-15b-5p抑制剂衰减。 MiR-15B-5P通过AKT路径下调VEGF信号传导;因此，miR-15b-5p RNA抑制剂可以是用于心血管疾病的可能治疗剂。

著录项

  • 来源
  • 作者

    Wu Wenfa; Zhou Taolong; Yu Huiwen; Jiang Zuofeng; Deng Wanqing; Zhang Suping; Liu Bohui; Wei Jianrui;

  • 作者单位

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

    Jinan Univ Guangzhou Red Cross Hosp Dept Cardiovasc Med Med Coll Guangzhou 510220 Guangdong Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 计量学;
  • 关键词

    Endothelial Cells; MicroRNA; VEGF; Apoptosis; Proliferation; Angiogenesis;

    机译：内皮细胞;microRNA;VEGF;凋亡;增殖;血管生成;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号