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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Embryonic expression of GINS members in the development of the mammalian nervous system
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Embryonic expression of GINS members in the development of the mammalian nervous system

机译：杜松子蛋白成员在哺乳动物神经系统发育中的胚胎表达

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The GINS (Go, Ichi, Nii, and San) complex contains four protein subunits (PSF1, PSF2, PSF3, and SLD5) and has been identified as a factor essential for the initiation and elongation stages of the DNA replication process. A previous study indicated that PSF2 participated in the developing central nervous system (CNS) of Xenopus laevis. However, the expression and function of GINS members in the mammalian developing nervous system remains unclear. Here, we examined the expression of GINS members in mice during nervous system development via immunofluorescence staining. At the beginning of neural development, PSF1 and SLD5 were highly expressed in neuroepithelial stem cells (NSCs) of the inner surface of neural tube (NT) and overlapped with proliferation marker Ki67. After entering the mid- and late-phase of neural development, PSF1 and SLD5 changed their regions of expression. These genes were highly expressed in dorsal root ganglion (DRG) progenitors, but they showed no overlap with Ki67 positive cells. Instead, a reduction of SLD5 expression promoted neuronal differentiation and maturation in the late-phase. PSF2 and PSF3 showed no tissue-specificity. PSF2 was constitutively and highly expressed whereas PSF3 was expressed at very low levels during neural development. In this study, we demonstrated variations in proteins and expression regions of the GINS members during mammalian CNS development and revealed a correlation between GINS expression and cell proliferation. Furthermore, we have suggested a novel function of GINS member SLD5, which regulates the differentiation of neural stem/progenitors.
机译：谷蛋白（GO，Ichi，NiI和San）复合物包含四种蛋白质亚基（PSF1，PSF2，PSF3和SLD5），并且已被鉴定为DNA复制过程的起始和伸长级的因子。先前的研究表明，PSF2参与了Xenopus Laevis的中枢神经系统（CNS）。然而，哺乳动物发育神经系统中杜松子蛋白构件的表达和功能仍然尚不清楚。在这里，我们在通过免疫荧光染色期间检查了神经系统开发期间小鼠中的胶林蛋白成员的表达。在神经发育开始时，PSF1和SLD5在神经管（NT）内表面的神经头脑干细胞（NSCs）中高度表达，并与增殖标记Ki67重叠。在进入神经发育中期和后期阶段后，PSF1和SLD5改变了他们的表达区域。这些基因在背根神经节（DRG）祖细胞中高度表达，但它们没有与Ki67阳性细胞的重叠重叠。相反，SLD5表达的降低促进了后期的神经元分化和成熟。 PSF2和PSF 3显示没有组织特异性。 PSF2组成型和高表达，而PSF 3在神经发育期间在非常低的水平下表达。在这项研究中，我们在哺乳动物CNS发育期间展示了杜松胶质蛋白和表达区域的变化，并揭示了谷蛋白表达和细胞增殖之间的相关性。此外，我们已经建议了GINS构件SLD5的新功能，其调节神经茎/祖细胞的分化。

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