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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Vascular endothelial growth factor influences migration and focal adhesions, but not proliferation or viability, of human neural stem/progenitor cells derived from olfactory epithelium
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Vascular endothelial growth factor influences migration and focal adhesions, but not proliferation or viability, of human neural stem/progenitor cells derived from olfactory epithelium

机译：血管内皮生长因子影响来自嗅觉上皮的人神经茎/祖细胞的迁移和局灶性粘连，但不增殖或活力

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摘要

In humans, new neurons are continuously added in the olfactory epithelium even in the adulthood. The resident neural stem/progenitor cells (hNS/PCs-OE) in the olfactory epithelium are influenced by several growth factors and neurotrophins. Among these modulators the vascular endothelial growth factor (VEGF) has attracted attention due its implicated in cell proliferation, survival and migration of other type of neural/stem progenitor cells. Interestingly, VEGFr2 receptor expression in olfactory epithelium has been described in amphibians but not in humans. Here we show that VEGFr is expressed in the hNS/PCs-OE. We also investigated the effect of VEGF on the hNS/PCs-OE proliferation, viability and migration in vitro. Additionally, pharmacological approaches showed that VEGF (0.5 ng/ml)-stimulated migration of hNS/PCs-OE was blocked with the compound DMH4, which prevents the activation of VEGFr2. Similar effects were found with the inhibitors for Rac (EHT1864) and p38MAPK (SB203850) proteins, respectively. These observations occurred with changes in focal adhesion contacts. However, no effects of VEGF on proliferation or viability were found in hNS/PCs-OE. Our results suggest that hNS/PCs-OE respond to VEGF involving VEGFr2, Rac and p38MAPK. (C) 2017 Elsevier Ltd. All rights reserved.
机译：在人类中，即使在成年期间，新的神经元也连续加入嗅性上皮中。嗅觉上皮中的驻留神经茎/祖细胞（HNS / PCS-OE）受几种生长因子和神经营养素的影响。在这些调节剂中，血管内皮生长因子（VEGF）由于其涉及其它类型的神经/茎祖细胞的细胞增殖，存活率和迁移而受到关注。有趣的是，在两栖动物中描述了嗅性上皮中的VEGFR2受体表达，但在两栖动物中已经描述但不在人类中。在这里，我们表明VEGFR在HNS / PCS-OE中表达。我们还研究了VEGF对体外HNS / PCS-OE增殖，活力和移液的影响。另外，药理学方法显示HNS / PCS-OE的VEGF（0.5ng / ml）刺激的迁移用化合物DMH4封闭，这防止了VEGFR2的活化。对于RAC（EHT1864）和P38MAPK（SB203850）蛋白的抑制剂，分别发现了类似的效果。这些观察结果发生在局灶性粘合触点的变化中。然而，在HNS / PCS-OE中发现VEGF对VEGF对增殖或活力的影响。我们的研究结果表明，HNS / PCS-OE响应VEGFR2，RAC和P38MAPK的VEGF。 （c）2017 Elsevier Ltd.保留所有权利。

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