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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >GLAST (GLutamate and ASpartate Transporter) in human prefrontal cortex; interactome in healthy brains and the expression of GLAST in brains of chronic alcoholics
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GLAST (GLutamate and ASpartate Transporter) in human prefrontal cortex; interactome in healthy brains and the expression of GLAST in brains of chronic alcoholics

机译：在人前额叶皮质中的凝血（谷氨酸和天冬氨酸转运蛋白）; 健康脑中的蛋白酶和慢性酗酒脑大脑的表达

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We have analysed post-mortem samples of prefrontal cortex from control and alcoholic human brains by the technique of Western blotting to estimate and compare the expressions of glutamate transporter GLAST (Excitatory Amino Acid Transporter One; EAAT1). Furthermore, using the non-alcoholic prefrontal cortex and custom-made GLAST (EAAT1) antibody we determined GLAST (EAAT1) "interactome" i.e. the set of proteins selectively bound by GLAST (EAAT1). We found that GLAST (EAAT1) was significantly more abundant (about 1.6-fold) in the cortical tissue from alcoholic brains compared to that from non-alcoholic controls. The greatest increase in the level of GLAST (EAAT1) was found in plasma membrane fraction (2.2-fold). Additionally, using the prefrontal cortical tissue from control brains, we identified 38 proteins specifically interacting with GLAST (EAAT1). These can be classified as contributing to the cell structure (6 proteins; 16%), energy and general metabolism (18 proteins; 47%), neurotransmitter metabolism (three proteins; 8%), signalling (6 proteins: 16%), neurotransmitter storage/release at synapses (three proteins; 8%) and calcium buffering (two proteins; 5%). We discuss possible consequences of the increased expression of GLAST (EAAT1) in alcoholic brain tissue and whether or how this could disturb the function of the proteins potentially interacting with GLAST (EAAT1) in vivo. The data represent an extension of our previous proteomic and metabolomic studies of human alcoholism revealing another aspect of the complexity of changes imposed on brain by chronic long-term consumption of ethanol.
机译：我们通过蛋白质印迹技术分析了从对照和酒精人体脑中预先逆转皮层的后验鼠样本，以估计并比较谷氨酸转运蛋白凝血剂（兴奋性氨基酸转运蛋白; EAAT1）的表达。此外，使用非酒精前额叶皮质和定制的Glast（EAAT1）抗体，我们确定了Glast（EAAT1）“酰蛋白组”即选择性地由PLAST（EAAT1）结合的蛋白质组。与非酒精对照相比，我们发现Plast（EAAT1）在从酒精脑中的皮质组织中较高（约1.6倍）。在血浆膜馏分（2.2倍）中发现了Glast（EAAT1）水平的最大增加。另外，使用来自对照大脑的前额叶皮质组织，我们确定了38种蛋白质，特别是与Plast（EAAT1）相互作用。这些可以被归类为有助于细胞结构（6蛋白; 16％），能量和一般代谢（18个蛋白质; 47％），神经递质代谢（三种蛋白质; 8％），信号传导（6蛋白：16％），神经递质在突触（三种蛋白质; 8％）和钙缓冲（两种蛋白质; 5％）的储存/释放。我们讨论了在酒精性脑组织中增加了Glast（EAAT1）表达的可能后果，以及如何干扰蛋白质在体内潜在地相互作用的蛋白质的功能。该数据代表我们之前的人类酗酒的先前蛋白质组学和代谢组研究的延伸，揭示了通过乙醇的慢性长期消费对脑施加的变化复杂性的另一个方面。

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