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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Enhanced neurogenesis and possible synaptic reorganization in the piriform cortex of adult rat following kainic acid‐induced status epilepticus
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Enhanced neurogenesis and possible synaptic reorganization in the piriform cortex of adult rat following kainic acid‐induced status epilepticus

机译：在Kinain酸诱导的状态癫痫症后成年大鼠脑内皮质中的增强神经发生和可能的突触重组

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Epileptic seizure has been reported to enhance adult neurogenesis and induce aberrant synaptic reorganization in the human dentate gyrus in the hippocampal formation. However, adult neurogenesis in the extrahippocampal regions has not been well studied. To investigate seizure‐enhanced neurogenesis in the extrahippocampal regions, we performed histological and immunohistochemical as well as western blot analyses on the cerebrum of Sprague–Dawley rats ( n ?=?51, male, 7?weeks old, body weight 250–300?g) treated with intraperitoneal injection of kainic acid (KA, 10?mg/kg) to induce status epilepticus (SE) ( n ?=?36) or normal saline solution ( n ?=?15) followed by 5′‐bromo‐2‐deoxyuridine (BrdU) injection to label newborn cells. Even though severe neuronal damage was found in the piriform cortex of rats having SE, immunohistochemistry for double cortin (DCX) revealed an increase in the number of immature neurons in the piriform cortex. Double immunofluorescence staining demonstrated that DCX‐positive cells in the piriform cortex were positive for both BrdU and neuronal nuclear antigen. Immunohistochemistry and western blotting revealed increased expressions of synaptophysin and postsynaptic density protein 95 in the piriform cortex of rat having SE. These results suggested the enhanced neurogenesis and possible synaptic reorganization in the piriform cortex of the KA‐treated rat.
机译：据报道，癫痫癫痫发作以增强成年神经发生，并在海马形成中诱导人齿状旋转中的异常突触重组。然而，突然藏品区的成人神经发生尚未得到很好的研究。为了探讨癫痫淘汰地区的癫痫发作增强神经发生，我们对Sprague-Dawley大鼠的大脑进行组织学和免疫组织化学以及Western印迹分析（n？= 51，男性，7周龄，体重250-300？ g）用腹膜内注射Kinaineal酸（Ka，10×mg / kg）治疗，以诱导状态癫痫（n？=β36）或生理盐水溶液（n？=Δ15），然后是5'-溴 - 2-脱氧尿苷（BRDU）注射到标记新生儿细胞。尽管在具有SE的大鼠的粒状皮质中发现了严重的神经元损伤，但是双皮原（DCX）的免疫组织化学揭示了脑鞘皮质中未成熟神经元数的增加。双免疫荧光染色证明了吡喃和神经元核抗原的粒子皮质中的DCX阳性细胞。免疫组织化学和蛋白质印迹显示突触素和突触后密度蛋白质95在具有SE的大鼠粒子皮质中的表达式增加。这些结果表明了KA处理大鼠的芯片皮质中增强的神经发生和可能的突触重组。

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