Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18

Lai Ying; Choi Ucheor B.; Leitz Jeremy; Rhee Hong Jun; Lee Choongku; Altas Bekir; Zhao Minglei; Pfuetzner Richard A.; Wang Austin L.; Brose Nils; Rhee JeongSeop; Brunger Axel T.

首页> 外文期刊>Neuron >Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18

【24h】

Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18

机译：Munc13和Munc18的突触囊泡引发的分子机制

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Munc13 catalyzes the transit of syntaxin from a closed complex with Munc18 into the ternary SNARE complex. Here we report a new function of Munc13, independent of Munc18: it promotes the proper syntaxin/synaptobrevin subconfiguration during assembly of the ternary SNARE complex. In cooperation with Munc18, Munc13 additionally ensures the proper syntaxin/SNAP-25 subconfiguration. In a reconstituted fusion assay with SNAREs, complexin, and synaptotagmin, inclusion of both Munc13 and Munc18 quadruples the Ca2+-triggered amplitude and achieves Ca2+ sensitivity at near-physiological concentrations. In Munc13-1/2 double-knockout neurons, expression of a constitutively open mutant of syntaxin could only minimally restore neurotransmitter release relative to Munc13-1 rescue. Together, the physiological functions of Munc13 may be related to regulation of proper SNARE complex assembly.

机译：Munc13催化与闭合络合物的粘合素的运输与Munc18中的闭合复合物进入三元蛇形复合物。在这里，我们报告了Munc13的新功能，独立于Munc18：它促进了三元链条复合体的组装期间促进了适当的Syntaxin / Synaptobrevin子配置。与Munc18合作，MunC13还可确保适当的Syntaxin / Snap-25子配置。在具有捕获，复合素和Sysaptotagmin的重构融合测定中，包含Munc13和Munc18四重组Ca2 + - 触发幅度，并在接近生理浓度下实现Ca2 +敏感性。在Munc13-1 / 2双敲除神经元中，棘突蛋白的组成型突变体的表达只能相对于Munc13-1救援最低恢复神经递质释放。在一起，Munc13的生理功能可能与适当的圈套复杂组件的调节有关。

著录项

来源
《Neuron》 |2017年第3期|共27页
作者
Lai Ying; Choi Ucheor B.; Leitz Jeremy; Rhee Hong Jun; Lee Choongku; Altas Bekir; Zhao Minglei; Pfuetzner Richard A.; Wang Austin L.; Brose Nils; Rhee JeongSeop; Brunger Axel T.;
展开▼
作者单位

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Max Planck Inst Expt Med Dept Mol Neurobiol D-37075 Gottingen Germany;

Max Planck Inst Expt Med Dept Mol Neurobiol D-37075 Gottingen Germany;

Max Planck Inst Expt Med Dept Mol Neurobiol D-37075 Gottingen Germany;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

Max Planck Inst Expt Med Dept Mol Neurobiol D-37075 Gottingen Germany;

Max Planck Inst Expt Med Dept Mol Neurobiol D-37075 Gottingen Germany;

Stanford Univ Dept Mol &

Cellular Physiol Stanford CA 94305 USA;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类神经病学;
关键词

相似文献

外文文献
中文文献
专利

1. Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18 [J] . Lai Ying, Choi Ucheor B., Leitz Jeremy, Neuron . 2017,第3期

机译：Munc13和Munc18的突触囊泡引发的分子机制
2. Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming [J] . Yang Xiaoyu, Wang Shen, Sheng Yi, Nature structural & molecular biology . 2015,第7期

机译：MUN结构域打开的突触蛋白是Munc13在突触小泡引发中的功能的基础
3. Munc13 homology domain-1 in CAPS/UNC31 mediates SNARE binding required for priming vesicle exocytosis. [J] . Khodthong C, Kabachinski G, James DJ, Cell metabolism . 2011,第2期

机译：CAPS / UNC31中的Munc13同源域1介导了引发小泡胞吐作用所需的SNARE结合。
4. The synaptic vesicle cycle is governed by heterogeneous and macromolecular protein microdomains [C] . Carla Schmidt ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：突触囊泡循环受到异质和大分子蛋白质微脂的控制
5. Molecular Mechanisms Controlling Synaptic Vesicle Fusion [D] . Radoff, Daniel 2011

机译：控制突触囊泡融合的分子机制
6. Molecular mechanisms of synaptic vesicle priming by Munc13 and Munc18 [O] . Ying Lai, Ucheor B. Choi, Jeremy Leitz, -1

机译：Munc13和Munc18引发突触小泡的分子机制
7. RIM Proteins Activate Vesicle Priming by Reversing Autoinhibitory Homodimerization of Munc13 [O] . Deng Lunbin, Kaeser Pascal S., Xu Wei, 2011

机译：RIM蛋白通过逆转Munc13的自抑制同质化作用激活囊泡启动。
8. Amino Acid Neurotransmitters; Mechanisms of Their Uptake into Synaptic Vesicles [R] . Fykse, E. M. 1991

机译：氨基酸神经递质;它们吸收到突触囊泡的机制

Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18

摘要

著录项

相似文献

相关主题

期刊订阅