...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neuron >Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis
【24h】

Synaptojanin and Endophilin Mediate Neck Formation during Ultrafast Endocytosis

机译：Synaptojanin和内皮蛋白介导颈部形成颈吞咽症期间

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ultrafast endocytosis generates vesicles from the plasma membrane as quickly as 50 ms in hippocampal neurons following synaptic vesicle fusion. The molecular mechanism underlying the rapid maturation of these endocytic pits is not known. Here we demonstrate that synaptojanin-1, and its partner endophilin-A, function in ultrafast endocytosis. In the absence of synaptojanin or endophilin, the membrane is rapidly invaginated, but pits do not become constricted at the base. The 5-phosphatase activity of synaptojanin is involved in formation of the neck, but 4-phosphatase is not required. Nevertheless, these pits are eventually cleaved into vesicles; within a 30-s interval, synaptic endosomes form and are resolved by clathrin-mediated budding. Then synaptojanin and endophilin function at a second step to aid with the removal of clathrin coats from the regenerated vesicles. These data together suggest that synaptojanin and endophilin can mediate membrane remodeling on a millisecond timescale during ultrafast endocytosis.
机译：超速度内吞作用在突触囊泡融合后的海马神经元中从质膜中从质膜中产生囊泡。这些内肾性凹坑的快速成熟的分子机制是未知的。在这里，我们证明Synaptojanin-1及其合作伙伴内胆蛋白-A，在超快内吞作用中的功能。在没有Synaptojanin或内皮蛋白的情况下，膜快速陷入困境，但凹坑不会在基础上被限制。 Synaptojanin的5-磷酸酶活性参与形成颈部，但不需要4-磷酸酶。然而，这些凹坑最终将切割成囊泡;在30-S间隔内，突触内体形成并通过克拉林介导的萌芽来解决。然后突触janin和内托宾函数在第二步中以帮助从再生囊泡中除去克拉辛涂层。这些数据在一起表明Synaptojanin和Endophilin可以在超自粒细胞增生期间介导在毫秒秒的少量粒度上进行膜重塑。

著录项

  • 来源
    《Neuron》 |2018年第6期|共20页
  • 作者

    Watanabe Shigeki; Mamer Lauren Elizabeth; Raychaudhuri Sumana; Luvsanjav Delgermaa; Eisen Julia; Trimbuch Thorsten; Soehl-Kielczynski Berit; Fenske Pascal; Milosevic Ira; Rosenmund Christian; Jorgensen Erik M.;

  • 作者单位

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

    Johns Hopkins Univ Sch Med Dept Cell Biol Baltimore MD 21205 USA;

    Johns Hopkins Univ Sch Med Dept Cell Biol Baltimore MD 21205 USA;

    Columbia Univ Barnard Coll New York NY USA;

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

    Univ Med Ctr Gottingen European Neurosci Inst Synapt Vesicle Dynam D-37077 Gottingen Germany;

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

    Charite Univ Med Berlin NeuroCure Cluster Excellence Dept Neurophysiol Berlin Germany;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 神经病学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号