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首页> 外文期刊>Neuron >Visual Experience-Dependent Expression of Fn14 Is Required for Retinogeniculate Refinement
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Visual Experience-Dependent Expression of Fn14 Is Required for Retinogeniculate Refinement

机译:视源细化需要视觉体验依赖表达FN14

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摘要

Sensory experience influences the establishment of neural connectivity through molecular mechanisms that remain unclear. Here, we employ single-nucleus RNA sequencing to investigate the contribution of sensory-driven gene expression to synaptic refinement in the dorsal lateral geniculate nucleus of the thalamus, a region of the brain that processes visual information. We find that visual experience induces the expression of the cytokine receptor Fn14 in excitatory thalamocortical neurons. By combining electrophysiological and structural techniques, we show that Fn14 is dispensable for early phases of refinement mediated by spontaneous activity but that Fn14 is essential for refinement during a later,?experience-dependent period of development. Refinement deficits in mice lacking Fn14 are associated with functionally weaker and structurally smaller retinogeniculate inputs, indicating that Fn14 mediates both functional and anatomical rearrangements in response to sensory experience. These findings identify Fn14 as a molecular link between sensory-driven gene expression and vision-sensitive refinement in the brain.
机译:感官体验影响通过持续不清楚的分子机制来建立神经连接。在这里,我们使用单核RNA测序来研究感觉驱动的基因表达对丘脑背侧突出核的突触细化的贡献,该大脑的进程视觉信息的区域。我们发现视觉经验诱导细胞因子受体FN14在兴奋性炎症神经元中的表达。通过结合电生理和结构技术,我们表明FN14可分配用于自发活动介导的细化的早期阶段,但是FN14在后续改进的情况下是必不可少的?经验依赖性发展期。缺乏Fn14的小鼠中的细化缺陷与功能较弱和结构较小的视黄胶输入相关,表明FN14响应感觉体验鉴于感觉体验介断功能性和解剖学重排。这些发现将Fn14鉴定为脑中感觉驱动的基因表达和视觉敏感细化之间的分子链接。

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