• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>New biotechnology >The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments
【24h】

The presence of PHB granules in cytoplasm protects non-halophilic bacterial cells against the harmful impact of hypertonic environments

机译：细胞质中pHB颗粒的存在保护非嗜嗜嗜氧细菌细胞免受高渗环境的有害影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Numerous prokaryotes accumulate polyhydroxybutyrate (PHB) intracellularly as a storage material. It has also been proposed that PHB accumulation improves bacterial stress resistance. Cupriavidus necator and its PHB non-accumulating mutant were employed to investigate the protective role of PHB under hypertonic conditions. The presence of PHB granules enhanced survival of the bacteria after exposure to hypertonic conditions. Surprisingly, when coping with such conditions, the bacteria did not utilize PHB to harvest carbon or energy, suggesting that, in the osmotic upshock of C. necator, the protective mechanism of PHB granules is not associated with their hydrolysis. The presence of PHB granules influenced the overall properties of the cells, since challenged PHB-free cells underwent massive plasmolysis accompanied by damage to the cell membrane and the leakage of cytoplasm content, while no such effects were observed in PHB containing bacteria. Moreover, PHB granules demonstrated "liquid-like" properties indicating that they can partially repair and stabilize cell membranes by plugging small gaps formed during plasmolysis. In addition, the level of dehydration and changes in intracellular pH in osmotically challenged cells were less pronounced for PHB-containing cultures, demonstrating the important role of PHB for bacterial survival under hyperosmotic conditions. (C) 2017 Elsevier B.V. All rights reserved.
机译：许多原核生物将细胞内作为储存材料累积多羟基丁酸酯（PHB）。还提出了PHB积累改善了细菌胁迫性。使用Cupriavidus Necator及其PHB非积聚突变体来研究PHB在高渗条件下的保护作用。在暴露于高渗条件后，PHB颗粒的存在增强了细菌的存活。令人惊讶的是，在应对这些条件时，细菌没有利用PHB来收获碳或能量，表明，在渗透渗透的渗透剂中，PHB颗粒的保护机理与其水解无关。 PHB颗粒的存在影响了细胞的整体性质，因为挑战的PHB无细胞正在进行大量等离子体分解，伴随细胞膜损伤和细胞质含量的泄漏，而在含有细菌的PHB中没有观察到这种效果。此外，PHB颗粒证明了“液体状”性质，表明它们可以通过在等离子体溶解期间形成的小间隙来部分修复和稳定细胞膜。此外，对含磷培养物的渗透性挑战细胞中细胞内pH的脱水水平和细胞内pH的变化较小，证明了PHB在高骨质病症下对细菌存活的重要作用。 （c）2017 Elsevier B.v.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号