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The effect of glutaraldehyde cross-linker on structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat

机译:戊二醛交联剂对胶原骨软骨电纺垫结构和生物相容性特性的影响

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摘要

The collagen with glycosaminoglycans such as chondroitin sulfate can potentially be used in many soft tissue engineering applications since the scaffolds mimic the structure and biological function of native extracellular matrix (ECM). In this study, we designed woven and aligned collagen-chondroitin sulfate mats by electrospinning method. The electrospun mats cross-linked with glutaraldehyde vapor at different times, the cross-linked mats became more biostable and were resistant to collagenase degradation. Cellular results with corneal epithelial cells showed that bioviability was reduced in cross-linked mats with glutaraldehyde. Characteristics such as high surface area of nanofibers, biodegradability, and orientation of fibers may maximize cell-ECM interaction and promote tissue regeneration faster than other conventional scaffolds. The cross-linked collagen-chondroitin sulfate electrospun mats can be used in tissue engineering, especially in soft tissue regeneration.
机译:具有糖胺聚糖的胶原蛋白,例如软骨素硫酸酯,可以在许多软组织工程应用中使用,因为支架模仿天然细胞外基质(ECM)的结构和生物学功能。 在这项研究中,我们通过静电纺丝方法设计了编织和对准的胶原蛋白软骨素硫酸盐垫。 电纺垫在不同时间与戊二醛蒸气交联,交联垫变得更加可生物,耐胶原酶降解。 具有角膜上皮细胞的细胞结果表明,用戊二醛的交联垫中减少了可变性。 诸如纳米纤维的高表面积,生物降解性和纤维取向的特性可以使细胞-ECM相互作用最大化并促进比其他常规支架更快的组织再生。 交联的胶原蛋白 - 软骨素硫酸盐垫垫可用于组织工程,特别是在软组织再生中。

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