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首页> 外文期刊>Materials science & engineering, C. Materials for Biogical applications >Chitosan/gamma-poly(glutamic acid) scaffolds with surface-modified albumin, elastin and poly-L-lysine for cartilage tissue engineering
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Chitosan/gamma-poly(glutamic acid) scaffolds with surface-modified albumin, elastin and poly-L-lysine for cartilage tissue engineering

机译：壳聚糖/γ-聚（谷氨酸）支架具有表面改性白蛋白，弹性蛋白和聚-L-赖氨酸的软骨组织工程

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摘要

Cartilage has limited ability to self-repair due to the absence of blood vessels and nerves. The application of biomaterial scaffolds using biomimetic extracellular matrix (ECM)-related polymers has become an effective approach to production of engineered cartilage. Chitosan/gamma-poly(glutamic acid) (gamma-PGA) scaffolds with different mass ratios were prepared using genipin as a cross-linker and a freeze-drying method, and their surfaces were modified with elastin, human serum albumin (HSA) and poly-L-lysine (PLL). The scaffolds were formed through a complex between -NH3+ of chitosan and -COO- of gamma-PGA, confirmed by Fourier transform infrared spectroscopy, and exhibited an interconnected porous morphology in field emission scanning electron microscopy analysis. The prepared chitosan/gamma-PGA scaffolds, at a 3:1 ratio, obtained the required porosity (90%), pore size (>= 100 pm), mechanical strength (compressive strength > 4 MPa, Young's modulus > 4 MPa) and biodegradation (30-60%) for articular cartilage tissue engineering applications. Surface modification of the scaffolds showed positive indications with improved activity toward cell proliferation (deoxyribonucleic acid), cell adhesion and ECM (glycoaminoglycans and type II collagen) secretion of bovine knee chondrocytes compared with unmodified scaffolds. In caspase-3 detection, elastin had a higher inhibitory effect on chondrocyte apoptosis in vitro, followed by HSA, and then PLL We concluded that utilizing chitosan/gamma-PGA scaffolds with surface active biomolecules, including elastin, HSA and PLL, can effectively promote the growth of chondrocytes, secrete ECM and improve the regenerative ability of cartilaginous tissues. (C) 2017 Elsevier B.V. All rights reserved.
机译：由于没有血管和神经，软骨具有有限的自我修复能力。使用仿生物微米基质（ECM） - 相关聚合物的生物材料支架已成为生产工程软骨的有效方法。使用Genipin作为交联剂制备具有不同质量比的壳聚糖/γ-聚（谷氨酸）（γ-PGA）支架，作为交联剂和冷冻干燥方法，用弹性蛋白，人血清白蛋白（HSA）和它们的表面进行改性聚L-赖氨酸（PLL）。通过傅立叶变换红外光谱证实，通过-NH3 +的壳聚糖和-COO-CHAMA-PGA之间的复合物形成支架，并在现场发射扫描电子显微镜分析中表现出相互连接的多孔形态。制备的壳聚糖/γ-PGA支架，在3：1的比例下获得所需的孔隙率（90％），孔径（> =100μm），机械强度（抗压强度> 4MPa，杨氏模量> 4MPa）和具有关节软骨组织工程应用的生物降解（30-60％）。与未修饰的支架相比，支架的表面改性表明，具有改善的细胞增殖（脱氧核糖核酸），细胞粘附和ECM（甘油酰基甘油和II型胶原蛋白的II型胶原蛋白）分泌的阳性适应症。在Caspase-3检测中，Elastin对体外的软骨细胞凋亡具有更高的抑制作用，然后是HSA，然后我们得出结论，利用壳聚糖/γ-PGA支架与表面活性生物分子，包括弹性蛋白，HSA和PLL，可以有效地促进软骨细胞的生长，分泌ECM，提高软骨组织的再生能力。 （c）2017 Elsevier B.v.保留所有权利。

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