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首页> 外文期刊>North American Journal of Fisheries Management >Comparing Efficiency of American Fisheries Society Standard Snorkeling Techniques to Environmental DNA Sampling Techniques
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Comparing Efficiency of American Fisheries Society Standard Snorkeling Techniques to Environmental DNA Sampling Techniques

机译：与美国渔业社会标准浮潜技术对环境DNA采样技术的效率

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摘要

Analysis of environmental DNA (eDNA) is an emerging technique used to detect aquatic species through water sampling and the extraction of biological material for amplification. Our study compared the efficacy of eDNA methodology to American Fisheries Society (AFS) standard snorkeling surveys with regard to detecting the presence of rare fish species. Knowing which method is more efficient at detecting target species will help managers to determine the best way to sample when both traditional sampling methods and eDNA sampling are available. Our study site included three Navajo Nation streams that contained Navajo Nation Genetic Subunit Bluehead Suckers Catostomus discobolus and Zuni Bluehead Suckers C. discobolus yarrowi. We first divided the entire wetted area of streams into consecutive 100-m reaches and then systematically selected 10 reaches/stream for snorkel and eDNA surveys. Surface water samples were taken in 10-m sections within each 100-m reach, while fish presence was noted via snorkeling in each 10-m section. Quantitative PCR was run on each individual water sample in quadruplicate to test for the presence or absence of the target species. With eDNA sampling techniques, we were able to positively detect both species in two out of the three streams. Snorkeling resulted in positive detection of both species in all three streams. In streams where the target species were detected with eDNA sampling, snorkeling detected fish at 11-29 sites/stream, whereas eDNA detected fish at 3-12 sites/stream. Our results suggest that AFS standard snorkeling is more effective than eDNA for detecting target fish species. To improve our eDNA procedures, the amount of water collected and tested should be increased. Additionally, filtering water on-site may improve eDNA techniques for detecting fish. Future research should focus on standardization of eDNA sampling to provide a widely operational sampling tool.
机译：环境DNA（EDNA）的分析是一种新兴技术，用于通过水采样检测水生物种和萃取生物材料进行扩增。我们的研究比较了EDNA方法对美国渔业社会（AFS）标准浮潜的疗效以及检测稀有鱼类的存在。了解在检测到目标物种时更有效的方法将有助于管理人员在可以使用传统的采样方法和EDNA采样时确定样本的最佳方式。我们的学习网站包括三个纳瓦霍民族溪流，其中包含Navajo国家遗传亚基Bluehead吸盘Catostomus Discobolus和Zuni Bluehead吸盘C. Discobolus Yarrowi。我们首先将河流的整个湿润区域划分为连续100米到达，然后系统地选择10到呼吸管和EDNA调查的10到距离。在每个100m覆盖物内以10μm截面拍摄表面水样，而每10米截面通过浮潜则注意到鱼类存在。在每包中的每种水样上运行定量PCR以测试靶物种的存在或不存在。凭借EDNA采样技术，我们能够在三个流中的两个流中积极地检测两个物种。浮潜导致在所有三个流中的两种物种阳性检测。在用EDNA采样检测目标物种的溪流中，在11-29个位点检测到鱼类鱼类/溪流，而edna检测到3-12位点的鱼类。我们的结果表明，AFS标准浮潜比edna更有效地检测目标鱼类。为了改善我们的EDNA程序，应增加收集和测试的水量。此外，在现场过滤水可以改善edna技术用于检测鱼类。未来的研究应侧重于EDNA采样的标准化，以提供广泛的操作采样工具。

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