Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein

Lin Bingqian; Guan Zhichao; Song Yanling; Song Eunyeong; Lu Zifei; Liu Dan; An Yuan; Zhu Zhi; Zhou Leiji; Yang Chaoyong

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Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein

机译：具有压力表读数的横向流动测定，用于快速护理蛋白质的护理点检测

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Paper-based assays such as lateral flow assays are good candidates for portable diagnostics owing to their user-friendly format and low cost. In terms of analytical detection, lateral flow assays usually require dedicated instruments to obtain quantitative results. Here we demonstrate a lateral flow assay with handheld pressure meter readout for the rapid detection of disease-related protein with high sensitivity and selectivity. Based on the pressure change produced by the catalytic reaction of Pt nanoparticles related to the concentration of the target, a quantitative reaction platform was established. During the lateral flow assay, the Pt nanoparticles are aggregated in the test line to form a gray band by biomolecular recognition and finally convert the recognition signal into highly sensitive pressure readout for quantitative analysis. Without sophisticated instrumentation and complicated operations, the whole detection process can be completed within 20 minutes. The limit of detection for myoglobin (2.9 ng mL(-1) in diluted serum samples) meets the requirements of clinical monitoring. With the advantages of low cost, ease of operation, high sensitivity and selectivity, the method represents a versatile platform for point-of-care testing of disease biomarkers.

机译：由于其用户友好的格式和低成本，诸如横向流量测定的纸质测定是便携式诊断的良好候选者。就分析检测而言，横向流动测定通常需要专用仪器来获得定量结果。在这里，我们用手持式压力表读出横向流动测定，用于快速检测具有高灵敏度和选择性的疾病相关蛋白。基于通过与靶浓度的Pt纳米颗粒的催化反应产生的压力变化，建立了定量反应平台。在横向流动测定期间，Pt纳米颗粒在测试线中聚集以形成灰度带，通过生物分子识别形成灰度，并且最终将识别信号转换为高敏感的压力读数以进行定量分析。如果没有复杂的仪器和复杂的操作，整个检测过程可以在20分钟内完成。乳蛋白（2.9 ng ml（-1）在稀释的血清样品中检测极限符合临床监测的要求。凭借成本低，易于操作，高灵敏度和选择性，该方法代表了一种通用平台，用于疾病生物标志物的护理点测试。

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《Lab on a chip》 |2018年第6期|共6页
作者
Lin Bingqian; Guan Zhichao; Song Yanling; Song Eunyeong; Lu Zifei; Liu Dan; An Yuan; Zhu Zhi; Zhou Leiji; Yang Chaoyong;
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作者单位

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

PASSTECH Co Ltd Xiamen 361005 Peoples R China;

Shanghai Jiao Tong Univ Renji Hosp Inst Mol Med Sch Med Shanghai 200127 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

Xiamen Univ MOE Key Lab Spectrochem Anal &

Instrumentat Collaborat Innovat Ctr Chem Energy Mat State Key Key Lab Chem Biol Fujian Prov Coll Chem &

Chem En Xiamen 361005 Peoples R China;

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正文语种 eng
中图分类化学实验（实验化学）;生物化学;生物科学;化学;
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专利

1. Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein [J] . Lin Bingqian, Guan Zhichao, Song Yanling, Lab on a chip . 2018,第6期

机译：具有压力表读数的横向流动测定，用于快速护理蛋白质的护理点检测
2. Europium chelate-labeled lateral flow assay for rapid and multiple detection of beta-lactam antibiotics by the penicillin-binding protein [J] . Li Xiaogang, Pan Zhifeng, Li Manyu, Analytical methods . 2020,第28期

机译：铕螯合物标记的侧向流动测定，通过青霉素结合蛋白快速和多次检测β-内酰胺抗生素
3. A sensitive and specific lateral flow assay for rapid detection of antibodies against glycoprotein B of Aujeszky's disease virus [J] . Journal of Virological Methods . 2017,第期

机译：用于快速检测Aujeszky疾病病毒糖蛋白B抗体的敏感和特异性横向流动测定
4. Developing rapid, point-of-care, multiplex detection for use in lateral flow devices [C] . Rao RS, Albala JS, Lane SL, Smart Medical and Biomedical Sensor Technology III . 2005

机译：开发用于侧向流量设备的快速现场即时多重检测
5. Development of lateral flow assays for detection of health risk markers. [D] . Leung, Wing-man. 2005

机译：开发用于检测健康风险标志物的侧向流动测定法。
6. Letter to the Editor regarding Peto T; UK COVID-19 Lateral Flow Oversight Team: COVID-19: Rapid antigen detection for SARS-CoV-2 by lateral flow assay [O] . Jonathan J Deeks, Jacqueline Dinnes, Clare Davenport, 2021

机译：关于Peto T的编辑的信;英国Covid-19横向流动监督团队：Covid-19：SARS-COV-2的快速抗原检测通过侧向流动测定
7. Frontispiece: Rapid, Quantitative, and Ultrasensitive Point-of-Care Testing: A Portable SERS Reader for Lateral Flow Assays in Clinical Chemistry [O] . Vi Tran, Bernd Walkenfort, Matthias König, 2019

机译：正面：快速，定量和超细管理点测试：临床化学中的侧向流动测定的便携式SERS阅读器

Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein

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