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首页> 外文期刊>Lab on a chip >Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans
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Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans

机译：从第一个幼虫阶段自动化的高含量表型，直到Nematode Caenorhabditis elegans的成年

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摘要

The nematode Caenorhabditis elegans is increasingly used as a model for human biology. However, in vivo culturing platforms for C. elegans allowing high-content phenotyping during their life cycle in an automated fashion are lacking so far. Here, a multiplexed microfluidic platform for the rapid high-content phenotyping of populations of C. elegans down to single animal resolution is presented. Nematodes are (i) reversibly and regularly confined during their life inside tapered channels for imaging fluorescence signal expression and to measure their growth parameters, and (ii) allowed to freely move in microfluidic chambers, during which the swimming behavior was video-recorded. The obtained data sets are analyzed in an automated way and 19 phenotypic parameters are extracted. Our platform is employed for studying the effect of bacteria dilution, a form of dietary restriction (DR) in nematodes, on a worm model of Huntington's disease and demonstrates the influence of DR on disease regression.
机译：Nematode caenorhabditise elegans越来越多地用作人类生物学的模型。然而，在Vivo培养平台上为秀丽隐杆线虫，允许到目前为止，缺乏自动化时尚的生命周期内的高含量表型。这里，提出了一种多路复用的微流体平台，用于快速高含量表型的C.杆状杆菌群体的快速高含量表型下降到单个动物分辨率。线虫（i）在锥形通道内可逆且经常被限制在锥形通道内，用于成像荧光信号表达，并测量它们的生长参数，并且（II）允许在微流体室中自由移动，在此期间游泳行为是视频记录的。通过自动化的方式分析所获得的数据集，提取19个表型参数。我们的平台用于研究细菌稀释的效果，在Nematodes的蠕虫模型中，对亨廷顿疾病的蠕虫模型进行研究，并展示了疾病回归博士的影响。

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