Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

Zhao Wujun; Liu Yang; Jenkins Brittany D.; Cheng Rui; Harris Bryana N.; Zhang Weizhong; Xie Jin; Murrow Jonathan R.; Hodgson Jamie; Egan Mary; Bankey Ana; Nikolinakos Petros G.; Ali Haythem Y.; Meichner Kristina; Newman Lisa A.; Davis Melissa B.; Mao Leidong

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Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

机译：肿瘤抗原无关和细胞大小变异 - 可行性循环肿瘤细胞的可包容性富集

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Isolation of circulating tumor cells (CTCs) from blood provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancer. The roles and clinical values of CTCs are under intensive investigation, yet most studies are limited by technical challenges in the comprehensive enrichment of intact and viable CTCs with minimal white blood cell (WBC) contamination. Here, we report a novel method based on contrast of cell magnetization in biocompatible ferrofluids (a colloidal magnetic nanoparticle suspension), termed as integrated ferrohydrodynamic cell separation (iFCS), that enriches CTCs in a tumor antigen-independent and cell size variation-inclusive manner, achieves a high throughput (12 mL h(-1)), high recovery rate (99.08% at down to similar to 10 cells per mL spike ratio), and low WBC contamination (533 cells for every one milliliter blood processed) and is biocompatible. This method will enable large cohort research to define the clinical and diagnostic value of CTC subtypes.

机译：血液中循环肿瘤细胞（CTC）的分离为转移性癌症的基本理解，诊断和预后提供了微创替代品。 CTCS的作用和临床价值在密集调查中，大多数研究受到在最小的白细胞（WBC）污染的完整和可行性CTC的综合性和活性CTC中的技术挑战的限制。在这里，我们报告了一种基于生物相容性铁物磁性流体（胶体磁性纳米粒子悬浮液）中细胞磁化的对比的新方法，称为集成的厌氧动力学细胞分离（IFCs），其在肿瘤抗原无关和细胞尺寸变异 - 包容性的肿瘤中富集CTC ，达到高通量（12mL H（-1）），高回收率（下降至相似的99.08％至每mL峰值比例），以及低WBC污染（每一个毫升血液处理的533个细胞），是生物相容性。该方法将使大型队列研究能够定义CTC亚型的临床和诊断值。

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来源
《Lab on a chip》 |2019年第10期|共17页
作者
Zhao Wujun; Liu Yang; Jenkins Brittany D.; Cheng Rui; Harris Bryana N.; Zhang Weizhong; Xie Jin; Murrow Jonathan R.; Hodgson Jamie; Egan Mary; Bankey Ana; Nikolinakos Petros G.; Ali Haythem Y.; Meichner Kristina; Newman Lisa A.; Davis Melissa B.; Mao Leidong;
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作者单位

Univ Georgia Dept Chem Athens GA 30602 USA;

Univ Georgia Dept Chem Athens GA 30602 USA;

Univ Georgia Dept Genet Athens GA 30602 USA;

Univ Georgia Sch Elect &

Comp Engn Athens GA 30602 USA;

Auburn Univ Dept Chem Engn Auburn AL 36830 USA;

Univ Georgia Dept Chem Athens GA 30602 USA;

Univ Georgia Dept Chem Athens GA 30602 USA;

Augusta Univ Univ Georgia Med Partnership Dept Med Athens GA 30602 USA;

Univ Canc &

Blood Ctr LLC Athens GA 30607 USA;

Univ Canc &

Blood Ctr LLC Athens GA 30607 USA;

Univ Canc &

Blood Ctr LLC Athens GA 30607 USA;

Univ Canc &

Blood Ctr LLC Athens GA 30607 USA;

Henry Ford Hlth Syst Dept Hematol &

Oncol Detroit MI 48202 USA;

Univ Georgia Coll Vet Med Dept Pathol Athens GA 30602 USA;

Henry Ford Hlth Syst Dept Surg Detroit MI 48202 USA;

Univ Georgia Dept Genet Athens GA 30602 USA;

Univ Georgia Sch Elect &

Comp Engn Athens GA 30602 USA;

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原文格式 PDF
正文语种 eng
中图分类化学实验（实验化学）;生物化学;生物科学;化学;
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专利

1. Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells [J] . Zhao Wujun, Liu Yang, Jenkins Brittany D., Lab on a chip . 2019,第10期

机译：肿瘤抗原无关和细胞大小变异 - 可行性循环肿瘤细胞的可包容性富集
2. Microfluidic chip combined with magnetic-activated cell sorting technology for tumor antigen-independent sorting of circulating hepatocellular carcinoma cells [J] . Pediatric transplantation. . 2019,第S1期

机译：微流体芯片结合磁性活性细胞分选技术，用于肿瘤抗原独立于循环肝细胞癌细胞的抗抗原分选
3. Fluidic Multivalent Membrane Nanointerface Enables Synergetic Enrichment of Circulating Tumor Cells with High Efficiency and Viability [J] . Lingling Wu, Hongming Ding, Xin Qu, Journal of the American Chemical Society . 2020,第10期

机译：流体多价膜纳米接口实现高效循环和循环肿瘤细胞的协同富集。
4. Ultra-High Throughput Enrichment of Viable Circulating Tumor Cells [C] . B.L. Khoo, M.E. Warkiani, Guofeng Guan International Conference on Biomedical Engineering . 2014

机译：可行循环肿瘤细胞的超高产量富集
5. Modeling and simulation of circulating tumor cells in flow. Part I: Low-dimensional deformation models for circulating tumor cells in flow. Part II: Procoagulant circulating tumor cells in flow [D] . Lee, Angela Meeyoun. 2013

机译：流动流动循环肿瘤细胞的建模与仿真。第一部分：用于循环肿瘤细胞流动的低维变形模型。第II部分：流动中的促凝血循环肿瘤细胞
6. Correction: Clinical Validation of an Ultra High-Throughput Spiral Microfluidics for the Detection and Enrichment of Viable Circulating Tumor Cells [O] . 2010

机译：更正：用于检测和富集活循环肿瘤细胞的超高通量螺旋微流控技术的临床验证
7. ClearCell® FX, a label‐free microfluidics technology for enrichment of viable circulating tumor cells [O] . Yifang Lee, Guofeng Guan, Ali Asgar Bhagat 2018

机译：ClearCell®FX，一种免费的微流体技术，用于富集可行的循环肿瘤细胞

Tumor antigen-independent and cell size variation-inclusive enrichment of viable circulating tumor cells

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