Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum Penicillium chrysogenum as a member of polysaccharide lyase family 26

Kunishige Yuika; Iwai Marin; Nakazawa Masami; Ueda Mitsuhiro; Tada Toshiji; Nishimura Shigenori; Sakamoto Tatsuji

首页> 外文期刊>FEBS letters. >Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum Penicillium chrysogenum as a member of polysaccharide lyase family 26

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Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum Penicillium chrysogenum as a member of polysaccharide lyase family 26

机译：来自青霉植物植物植物中的Exo-rhamnogalactuRonan碱酶的晶体结构作为多糖裂解酶系列的成员26

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Exo‐rhamnogalacturonan lyase from Penicillium chrysogenum 31B (Pc RGLX ) was recently classified as a member of polysaccharide lyase ( PL ) family 26 along with hypothetical proteins derived from various organisms. In this study, we determined the crystal structure of Pc RGLX as the first structure of a member of this family. Based on the substrate‐binding orientation and substrate specificity, Pc RGLX is an exo‐type PL that cleaves rhamnogalacturonan from the reducing end. Analysis of Pc RGLX ‐complex structures with reaction products indicate that the active site possesses an L‐shaped cleft that can accommodate galactosyl side chains, suggesting side‐chain‐bypassing activity in Pc RGLX . Furthermore, we determined the residues critical for catalysis by analyzing the enzyme activities of inactive variants.

机译：从青霉钙瘤31b（pc rglx）的Exo-rhamnogalactulanan碱酶最近被分类为多糖裂解酶（Pl）家族26的成员以及来自各种生物的假设蛋白质。在这项研究中，我们确定了PC RGLX的晶体结构作为本系成员的第一结构。基于基质结合取向和底物特异性，PC RGLX是从还原末端切割鼠李杆菌属植物的外壳型PL。用反应产物的PC rglx -complex结构的分析表明，活性位点具有L形裂缝，其可以容纳半乳糖基侧链，表明PC RGLX中的侧链旁路活性。此外，我们通过分析惰性变体的酶活性来确定对催化的残留物至关重要。

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来源
《FEBS letters.》 |2018年第8期|共11页
作者
Kunishige Yuika; Iwai Marin; Nakazawa Masami; Ueda Mitsuhiro; Tada Toshiji; Nishimura Shigenori; Sakamoto Tatsuji;
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作者单位

Division of Applied Life SciencesOsaka Prefecture UniversitySakai Japan;

Division of Applied Life SciencesOsaka Prefecture UniversitySakai Japan;

Division of Applied Life SciencesOsaka Prefecture UniversitySakai Japan;

Division of Applied Life SciencesOsaka Prefecture UniversitySakai Japan;

Department of Biological ScienceOsaka Prefecture UniversitySakai Japan;

Division of Applied Life SciencesOsaka Prefecture UniversitySakai Japan;

Division of Applied Life SciencesOsaka Prefecture UniversitySakai Japan;

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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
crystal structure; exo‐rhamnogalacturonan lyase; polysaccharide lyase family 26;

机译：晶体结构;Exo-rhamnogalacturonan裂解酶;多糖裂解酶家庭26;

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1. Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum Penicillium chrysogenum as a member of polysaccharide lyase family 26 [J] . Kunishige Yuika, Iwai Marin, Nakazawa Masami, FEBS letters. . 2018,第8期

机译：来自青霉植物植物植物中的Exo-rhamnogalactuRonan碱酶的晶体结构作为多糖裂解酶系列的成员26
2. Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum as a member of polysaccharide lyase family 26 [J] . Yuika Kunishige, Marin Iwai, Masami Nakazawa, FEBS Letters . 2018,第8期

机译：产黄青霉作为多糖裂解酶家族成员的外鼠李糖半乳糖醛酸裂解酶的晶体结构26
3. Molecular characterization of a Penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins [J] . Iwai Marin, Kawakami Takuya, Ikemoto Takeshi, Applied Microbiology and Biotechnology . 2015,第20期

机译：产黄青霉外鼠李糖半乳糖醛酸聚糖裂解酶的分子特征，其结构不同于其他多糖裂解酶家族蛋白
4. Antioxidant properties of halotolerant Penicillium chrysogenum var. chrysogenum secondary metabolites [C] . Canturk Zerrin, Dikmen Miris, Ozturk Nilgun, IEEE International Conference on E-Health and Bioengineering . 2013

机译：卤代青霉变种的抗氧化性能。产黄次生代谢物
5. Isolation and characterization of secondary metabolites from Canadian indoor strains of Penicillium brevicompactum and Penicillium chrysogenum. [D] . De La Campa, Regina. 2005

机译：来自加拿大室内紧凑型青霉菌和产黄青霉菌的次生代谢产物的分离和鉴定。
6. Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168 a member of polysaccharide lyase family 11 [O] . Akihito Ochiai, Masayuki Yamasaki, Takafumi Itoh, 2006

机译：枯草芽孢杆菌菌株168（多糖裂解酶家族11的成员）中鼠李糖半乳糖醛酸聚糖裂解酶YesW的结晶和初步X射线分析
7. Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11 [O] . Ochiai, Akihito, Yamasaki, Masayuki, Itoh, Takafumi, 2006

机译：枯草芽孢杆菌菌株168（多糖裂解酶家族11的成员）中鼠李糖半乳糖醛酸聚糖裂解酶YesW的结晶和初步X射线分析

Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum Penicillium chrysogenum as a member of polysaccharide lyase family 26

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