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Genetically engineering Crambe abyssinicaA potentially high-value oil crop for salt land improvement

机译：基因工程克拉姆斯索尼察潜在的盐土地改善的高价值油作物

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Crambe abyssinica (crambe) is a new industrial oil crop that can grow on saline soil and tolerates salty water irrigation. Genetically engineered crambe in which the seed-oil composition is manipulated for more erucic acid and less polyunsaturated fatty acid (PUFA) would be highly beneficial to industry. In this research, lysophosphatidic acid acyltransferase 2 RNA interference (CaLPAT2-RNAi) was introduced into the crambe genome to manipulate its oil composition. The result showed in comparison with wild type, CaLPAT2-RNAi could significantly reduce linoleic and linolenic acid content, simultaneously increasing erucic acid content. Systematic metabolism engineering was then carried out to further study CaLPAT2-RNAi, combined with the overexpression of Brassica napusfatty acid elongase (BnFAE), Limnanthes douglasii LPAT (LdLPAT), and RNAi of endogenous fatty acid desaturase 2 (CaFAD2-RNAi). Oil composition analysis on the tranformants' seeds showed that (a) with CaFAD2-RNAi, PUFA content could be dramatically decreased, in comparison with BnFAE+LdLPAT+CaFAD2-RNAi, and BnFAE+LdLPAT+CaFAD2-RNAi+CaLPAT2-RNAi seeds showed lower linolenic acid content; (b) BnFAE+LdLPAT+CaFAD2-RNAi+CaLPAT2-RNAi could increase the erucic acid content in crambe seed oil from less than 66.6% to 71.6%, whereas the highest erucic acid content of BnFAE+LdLPAT+CaFAD2-RNAi was 79.2%; (c) although the four-gene combination could not increase the erucic acid content of seed oil to a higher level than the others, it led to increased carbon resource deposited into C22:1 and C18:1 moieties and lower PUFA. Summarily, the present research indicates that suppression of LPAT2 is a new, promising strategy for seed-oil biosynthesis pathway engineering, which would increase the value of crambe oil.
机译：Crambe Abyssinica（Crambe）是一种新的工业油作物，可在盐渍土上生长，并耐受咸水灌溉。基因工程克拉姆克拉姆，其中种子 - 油组合物被操纵以进行更多型芥酸，并且较少的多不饱和脂肪酸（PUFA）对工业具有高度有益的。在该研究中，将溶血磷脂酸酰基转移酶2引入CRAMBE基因组中的RNA干扰（CALPAT2-RNAi）以操纵其油组合物。结果表明，与野生型相比，Calpat2-RNAi可以显着降低亚油和亚麻酸含量，同时增加芥酸含量。然后进行系统的代谢工程进一步研究CALPAT2-RNAi，与甘蓝醛酸的过度表达联合芸苔的过表达（BNFAE），LIMNANTHES DOGLASII LPAT（LDLPAT）和内源性脂肪酸去饱和酶2的RNAi（CaFad2-RNAi）。散装种子的油组成分析显示（a）与Cafad2-RNAi，与BNFAE + LDLPAT + CaFad2-RNAi相比，PUFA含量可以显着降低，并且BNFae + Ldlpat + CaFad2-RNai + Calpat2-RNAi种子显示出来降低亚麻酸含量; （b）BNFAE + LDLPAT + CAFAD2-RNAI + CALPAT2-RNAI可以将CRAMBE种子油中的芥酸含量从小于66.6％增加至71.6％，而BNFAE + LDLPAT + CAFAD2-RNAi的最高核酸含量为79.2％ ; （c）虽然四基因组合不能将种子油的芥酸含量增加到更高水平，但它导致沉积在C22：1和C18：1部分和降低PUFA中的碳资源。概述，本研究表明，LPAT2的抑制是种子油生物合成途径工程的一种新的，有希望的策略，这将增加Crambe油的价值。

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