A label‐free fluorescence method for detection of        <i >ureCureC        gene and diagnosis of        <fc >          <fi>Helicobacter pylori</fi>        </fc>          <fi >Helicobacter pylori</fi>Helicobacter pylori                infection

Zhang Leiyi; Cao Ding; Tang Tenglong; Zuo Zhongkun; Huang Jiangsheng; Duan Lunxi

摘要

Abstract > The feasibility of using a polymerase chain reaction (PCR)‐based label‐free DNA sensor for the detection of <fc> <fi>Helicobacter pylori</fi> </fc> is investigated. In particular, <fc>H. pylori</fc> ureC gene, a specific <fc> <fi>H. pylori</fi> </fc> nucleic acid sequence, was selected as the target sequence. In the presence of ureC gene, the target DNA could be amplified to dsDNA with much higher detectable levels. After added the SYBR green I (SGI), the sensing system could show high fluorescence. Thus, the target DNA can be detected by monitoring the change of fluorescence intensity of sensing system. The clinical performance of this method was determined by comparing it with another conventional technique urea breath test (UBT). The result also showed good distinguishing ability between negative and positive patient, which was in good agreement with that obtained by the UBT. It suggests that the label‐free fluorescence‐based method is more suitable for infection confirmation test of <fc> <fi>H. pylori</fi> </fc> . This approach offers great potential for simple, sensitive and cost‐effective identification of <fc> <fi>H. pylori</fi> </fc> infection. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >使用聚合酶链反应（PCR）的可行性 - 基于无标记的DNA传感器研究了检测<FC> <FI>幽门螺杆菌</ Fi> </ Fc>。特别是 <fc> h。幽门螺杆菌</ i>基因，特定的<fc> <fi> h。幽门螺杆菌</ fi> </ fc>核酸序列作为靶序列。在 UREC </ I>基因存在下，可以以更高的可检测水平扩增靶DNA。在添加Sybr Green I（SGI）之后，传感系统可以显示出高荧光。因此，可以通过监测传感系统的荧光强度的变化来检测靶DNA。通过将其与另一种常规技术尿素呼吸试验（UBT）进行比较来确定该方法的临床性能。结果也表现出负面和阳性患者之间的良好显着能力，这与UBT获得的良好一致。它表明，无标记的基于荧光的方法更适合于<Fc> <Fi> H的感染确认试验。 Pylori </ Fi> </ Fc>。这种方法可以为<FC> <FI> H的简单，灵敏和经济高效的识别提供很大的潜力。幽门螺杆菌</ fi> </ fc>感染。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26441/'>《Luminescence: The journal of biological and chemical luminescence》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共6页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Leiyi&option=202" target="_blank" rel="nofollow">Zhang Leiyi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cao Ding&option=202" target="_blank" rel="nofollow">Cao Ding;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tang Tenglong&option=202" target="_blank" rel="nofollow">Tang Tenglong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zuo Zhongkun&option=202" target="_blank" rel="nofollow">Zuo Zhongkun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Jiangsheng&option=202" target="_blank" rel="nofollow">Huang Jiangsheng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Duan Lunxi&option=202" target="_blank" rel="nofollow">Duan Lunxi;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Minimally Invasive SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha Hunan P. R. China;</p> <p>Department of Minimally Invasive SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha Hunan P. R. China;</p> <p>Department of Minimally Invasive SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha Hunan P. R. China;</p> <p>Department of Minimally Invasive SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha Hunan P. R. China;</p> <p>Department of Minimally Invasive SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha Hunan P. R. China;</p> <p>Department of Minimally Invasive SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha Hunan P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1345.html" title="生物光学">生物光学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fluorescence&option=203" rel="nofollow">fluorescence;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Helicobacter pylori infection&option=203" rel="nofollow">Helicobacter pylori infection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=label‐free&option=203" rel="nofollow">label‐free;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ureC gene&option=203" rel="nofollow">ureC gene;</a> </p> <div class="translation"> 机译：荧光;幽门螺杆菌感染;无标签;UREC基因; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023986356.html">A label‐free fluorescence method for detection of <i >ureCureC gene and diagnosis of <fc > <fi>Helicobacter pylori</fi> </fc> <fi >Helicobacter pylori</fi>Helicobacter pylori infection</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Leiyi&option=202" 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dwidar N&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Dwidar N,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Helaly G&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Helaly G </a> <a href="/conference-foreign-274697/" target="_blank" rel="nofollow" class="tuijian_authcolor">Incorporating of the Australian Society for Biochemistry and Molecular Biology Combio .</a> <span>2009</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：幽门螺杆菌感染的分子诊断和CAGA和VACA基因存在的危险因素</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061692569.html">Analysis of mucin oligosaccharides in the rhesus monkey stomach after Helicobacter pylori infection and transcriptional modulation of outer membrane protein expression in Helicobacter pylori</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cooke, Cara Lea&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cooke, Cara Lea </a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：幽门螺杆菌感染后恒河猴胃粘蛋白寡糖分析及幽门螺杆菌外膜蛋白表达的转录调控</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/04000608522.html">Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cagA gene in gastric biopsy specimens.</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=A P Lage&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">A P Lage,</a> <a 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class="tuijian_auth tuijian_authcolor">A P Lage,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=E Godfroid&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">E Godfroid,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=A Fauconnier&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">A Fauconnier,</a> <span>1995</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：PCR幽门螺杆菌感染的诊断：与其他侵入性技术的比较和胃活组织检查标本中的CAGA基因检测</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201294239647.html">Creation of Virtual Wi-Fi Access Point and Secured Wi-Fi Pairing, through NFC</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oussama Stiti&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Oussama Stiti</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Othmen Braham&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Othmen Braham</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guy Pujolle&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Guy Pujolle</a> <span> <a href="/journal-cn-56342/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 通讯、网络与系统学国际期刊（英文） </a> </span> <span> . 2014</span><span>,第6期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_mobile-communications_thesis/0201237955626.html">基于运营商公共开放Wi-Fi网络的Wi-Fi Calling业务集成方法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=巫长征&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 巫长征</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=林文锋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,林文锋</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=关伟杰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,关伟杰</a> <span> <a href="/journal-cn-8801/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 移动通信 </a> </span> <span> . 2017</span><span>,第014期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-immunology_thesis/0201223457385.html">在真核细胞中表达的幽门螺杆菌UreC,OipA抗原性的检测及其对细胞的作用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张静&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张静</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=佘菲菲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,佘菲菲</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈豪&option=202" target="_blank" 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郑宇辉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 郑宇辉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨映红&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨映红</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=邱小文&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,邱小文</a> <span> <a href="/journal-cn-6499/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 福建医科大学学报 </a> </span> <span> . 2013</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-12332_thesis/02022617887.html">20个家庭118名成员中反复腹痛和无反复腹痛者的幽门螺杆菌感染和HLA—DQB1、DRB1等位基因频率分析</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄永坤&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 黄永坤</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李继梅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李继梅</a> <span> <a href="/conference-cn-12332/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十届全国微生态学学术会议 </a> <span> <span> . 2010</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314116439.html">评价幽门螺杆菌粪便抗原试验诊断儿童幽门螺杆菌感染的临床价值</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张冰峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张冰峰</a> <span> . 2005</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120108118207.html">Wi-Fi连接方法、终端、Wi-Fi设备、Wi-Fi连接控制方法和服务器</a> <b>[P]</b> . <span> 中国专利： CN105635097A </span> <span> . 2016-06-01</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061203498063.html">Wi-Fi网络连接方法、Wi-Fi控制器及Wi-Fi设备</a> <b>[P]</b> . <span> 中国专利： CN104412664B </span> <span> . 2019.10.18</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130450891686.html">Vaccine composition, use of a helicobacter pylori polypeptide flge or a modified form thereof, use of the same, vaccine composition to induce a protective immune response to helicobacter pylori infection, in vitro diagnostic process of helicobacter pylori infection, kit method for detecting helicobacter pylori infection in a mammal including man, and process for eliciting a protective immune response against helicobacter pylori infection in a mammal</a> <b>[P]</b> . <span> 外国专利：  BR9810026A </span> <span> . 2000-09-19</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：疫苗组合物，幽门螺杆菌多肽壁或其修饰形式的用途，其用途，疫苗组合物诱导对幽门螺杆菌感染的保护性免疫应答，幽门螺杆菌感染的体外诊断方法，用于检测幽门螺杆菌感染的试剂盒方法包括人在内的哺乳动物中的表达，以及引起针对幽门螺杆菌感染的保护性免疫应答的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130455383019.html">HELICOBACTER PYLORI-COLONIZED MONGOLIAN GERBIL, PROCESS FOR PREPARATION THE REOF. MEDIUM FOR SEPARATING HELICOBACTER PYLORI, HELICOBACTER PYLORI-TRANSFORMED MUNGO VADEL, METHOD OF MANUFACTURING THE SAME, HELICOBAKTER PILLARY SEPARATION MEDIUM, AND METHOD FOR SCREENING ANTI-AND METHOD OF SCREENING ANTI-HELICOBACTER PYLORI-ACTIVE SUBSTANCE BY USING THE SAME)</a> <b>[P]</b> . <span> 外国专利：  KR970700994A </span> <span> . 1997-03-17</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：幽门螺杆菌蒙古蒙古，准备的过程。用于分离幽门螺杆菌的培养基，经幽门螺杆菌转化的Mungo vadel，制造该培养基的方法，用于分离幽门螺杆菌的培养基，用于筛选抗卵菌的方法以及通过使用该物质筛选抗幽门螺杆菌活性物质的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130456593115.html">Helicobacter pylori reared Mongolian gerbil, method for producing the same, medium for Helicobacter pylori separation and method for screening anti Helicobacter pylori active substance</a> <b>[P]</b> . <span> 外国专利：  JPWO96/18291A1 </span> <span> . 1997-03-25</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：幽门螺杆菌饲养的蒙古沙鼠，其生产方法，幽门螺杆菌分离培养基和抗幽门螺杆菌活性物质的筛选方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023986356','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" 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A label‐free fluorescence method for detection of ureCureC gene and diagnosis of Helicobacter pylori Helicobacter pyloriHelicobacter pylori infection

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