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首页> 外文期刊>Limnology >Determination of dissolved and particulate thiols in Lake Biwa water and extracted fulvic acids by solid phase extraction followed by HPLC with fluorescence detection
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Determination of dissolved and particulate thiols in Lake Biwa water and extracted fulvic acids by solid phase extraction followed by HPLC with fluorescence detection

机译：用荧光检测用固相萃取测定琵琶湖水中溶解和颗粒硫醇，用固相萃取萃取富含氟酸

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摘要

Thiols are important antioxidants that can modulate the bioavailability and biogeochemistry of many soft metals, although their detection remains challenging in both their reduced (R-S) and oxidized (R-S-S-R) forms. Here, a modified biochemical method was applied to determine the levels of dissolved and particulate thiols in Lake Biwa water and extracted Lake Biwa fulvic acids obtained at various depths. This method involves the use of the reducing agent tris(2-carboxyethyl)phosphine and the fluorescent label 7-fluorobenzofurazan-4-sulfonic acid ammonium salt (SBD-F), followed by solid-phase extraction and HPLC with fluorescence detection. Dissolved cysteine (Cys) (2.0-6.0 nM), glutathione (GSH) (2.8-5.1 nM), and N-acetyl-l-cysteine (NAC) (1.6-4.2 nM) were detected throughout the water column but were broadly consistent at depths of 5-20 m. In contrast, abundant levels of particulate cysteine (1.3-3.5 x 10(2) nM) and glutathione (1.6-3.1 x 10(2) nM) were detected down to depths of 15 m. The particulate cysteine and glutathione were significantly covariant, and the ratios between them reflected the differences in the plankton community composition and availability of these compounds. This work also studied the concentrations of Cys, GSH and NAC in Lake Biwa fulvic acids (LBFAs) for the first time (at 0 m: cysteine, 0.8 nM; glutathione, 1.6 nM; NAC, 2.5 nM; at 10 m: cysteine, 1.4 nM; glutathione, 0.6 nM; NAC, 1.6 nM). The nanomolar to sub-nanomolar concentrations of the particulate and dissolved Cys, GSH and NAC in the lake indicates that these are an important class of ligands for chalcophile metals and may influence the distribution of plankton communities from the epilimnion to the hypolimnion of the lake.
机译：硫醇是重要的抗氧化剂，可以调节许多软金属的生物利用度和生物地球化学，尽管它们的检测在其还原（R-S）和氧化（R-S-S-R）形式中保持挑战性。这里，应用改性的生物化学方法以确定琵琶湖水中的溶解和颗粒状硫醇水平，并在各种深度中获得的湖泊琵琶湖富含酸盐。该方法涉及使用还原剂Tris（2-羧乙基）膦和荧光标记7-氟苯脲脲酰亚胺-4-磺酸铵盐（SBD-F），然后具有荧光检测的固相萃取和HPLC。在整个水柱中检测到溶解半胱氨酸（Cys）（2.0-6.0nm），谷胱甘肽（GSH）（2.8-5.1nm）和N-乙酰-1-半胱氨酸（NaC）（1.6-4.2nm），但广泛一致深度为5-20米。相比之下，检测到颗粒状半胱氨酸（1.3-3.5×10（2）纳米）和谷胱甘肽（1.6-3.1×10（2）nm）的水平，低至15μm。颗粒状半胱氨酸和谷胱甘肽是显着的协变性，它们之间的比率反映了浮游生物群落组成和这些化合物的可用性的差异。这项工作也首次研究了Cys，GSH和NAC的浓度（LBFAs）的浓度（在0 m：半胱氨酸，0.8nm;谷胱甘肽，1.6nm; Nac，2.5nm;在10米：半胱氨酸， 1.4 nm;谷胱甘肽，0.6nm; Nac，1.6nm）。湖中亚马尔的亚马尔·亚马尔浓度，GSH和NAc在湖中表明，这些是碳酸化合物金属的重要配体，并且可能影响来自湖泊的癫痫术中的Plankton群落的分布。

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