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首页> 外文期刊>Limnology >The genetic diversity and phylogeny of green microalgae?in the genus Choricystis (Trebouxiophyceae, Chlorophyta) in Lake Baikal
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The genetic diversity and phylogeny of green microalgae?in the genus Choricystis (Trebouxiophyceae, Chlorophyta) in Lake Baikal

机译:绿色微藻的遗传多样性和系统发育?在贝加尔湖(Trebouxiophyceae)属中(Trebouxiophyceae,叶绿藻)

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摘要

Green microalgae are of the utmost importance to aquatic ecosystems globally, as they are primary producers and major food sources for many organisms. Microalgae of the genus Choricystis are common in Lake Baikal, yet genetic diversity and phylogenetic relationships of the genus are not well known. Nuclear 18S rRNA, plastid rbcL and 16S rRNA gene sequences from open databases were used to investigate the relationships within the genus Choricystis and to specify the phylogeny and diversity of these microalgae in Lake Baikal. Sequences derived from both planktonic Choricystis and endosymbiotic Choricystis living within Baikal endemic sponges were analysed. Species delimitation was performed using bioinformatic methods. Analysis of the three genetic markers showed two major phylogenetic lineages in the genus Choricystis, with the highest genetic diversity observed in the rbcL gene analysis. Two different phylogenetic clusters included sequences of strains with the same species name, Choricystis parasitica (K. Brandt) Pr?schold & Darienko, raising the question of hidden diversity within this species. Choricystis algae in Lake Baikal are represented by three genetic variants, two of which are distinct from Choricystis parasitica. Analysis of nuclear and plastid genes in microalgae of the genus Choricystis showed higher genetic diversity than previously thought and a possible endemic speciation in Lake Baikal.
机译:Green Microalgae对全球水生生态系统至关重要,因为它们是许多生物的主要生产者和主要食物来源。在贝氏湖属植物湖中常见的微藻果园,但遗传多样性和属的遗传多样性和神经发育关系尚不清楚。核18S rRNA,塑性RBCL和16S rRNA基因序列来自开放数据库的序列探讨了Choricystis内的关系,并在贝加尔湖中指定这些微藻的系统发育和多样性。分析了植物植物含有植物毒素和患有鲈鱼特有的海绵状物内的胆囊炎和胚胎胆炎的序列。使用生物信息方法进行物种划界。三种遗传标记的分析显示了在醋毛湿病属中的两个主要系统发育谱系,在RBCL基因分析中观察到最高的遗传多样性。两个不同的系统发育簇包括具有相同物种名称的菌株序列,Choricystis parasitica(K.Bardt)Pr?Schold&Darienko,提出了本物种内隐藏多样性的问题。贝加尔湖的Choricystis藻类由三种遗传变异表示,其中两种遗传变异是含有胆炎寄生虫的。蜂清液中微藻核和体层基因的分析表明,贝加尔湖遗传多样性高度遗传多样性和可能的​​地方性物质。

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