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首页> 外文期刊>Limnology and oceanography, methods >A novel fracturing device to observe the gut contents of copepod nauplii using a scanning electron microscope
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A novel fracturing device to observe the gut contents of copepod nauplii using a scanning electron microscope

机译：使用扫描电子显微镜观察Copepod Nauplii的肠道内容物的新型压裂装置

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摘要

We introduce a novel fracturing device of high accuracy for scanning electron microscopy for the identification of in situ food items in copepod nauplii. The device requires a very small amount of water and sample, which is frozen on the device. The ice is then physically fractured with the included sample, and sublimated to dry. Copepod nauplii of Acartia steueri were fractured with arbitrary cross-sections using this device, and their digestive tracts were observed. Cyanobacteria, diatoms, bacteria, and flagellates were observed in the guts. Elemental analysis of a fecal pellet in the hind gut of a copepod nauplius detected silicon. The accuracy of the device was tested and distribution of fracturing errors showed a normal distribution; the cumulative frequency between -50 mu m and 50 mu m was 0.81. The accuracy indicates that the device can be widely applied to other microscopic organisms such as barnacle nauplii, starfish larvae, and sea urchin larvae.
机译：我们介绍了一种高精度的新型压裂装置，用于扫描电子显微镜，以鉴定在Copepod Nauplii中的原位食品。 该装置需要非常少量的水和样品，在装置上冷冻。 然后将冰与包含的样品进行物理裂缝，并升华干燥。 Acartia Steueri的CopePod Nauplii用使用该装置的任意横截面进行裂缝，并观察到它们的消化道。 在肠道中观察到蓝细菌，硅藻，细菌和鞭毛和鞭毛。 Copepod Nauplius检测到硅的后肠道粪便颗粒的元素分析。 测试装置的准确性并分布压裂误差显示正态分布; 累积频率在-50μm和50μm之间为0.81。 精度表明该装置可广泛应用于其他微观生物，例如藤鸟，海星幼虫和海胆幼虫。

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