...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Aerosol Science and Technology: The Journal of the American Association for Aerosol Research >Effective Collection of Airborne Micro-Organisms by Direct Impaction on Silicone Sealants-Comparison of Different Adherent Surfaces
【24h】

Effective Collection of Airborne Micro-Organisms by Direct Impaction on Silicone Sealants-Comparison of Different Adherent Surfaces

机译：直接撞击有机硅密封胶可有效收集机载微生物-不同粘附表面的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

For effective sampling of airborne micro-organisms different techniques such as impingement, filtration and impaction are commonly used. Impaction is the method which allows best for direct collection on adhesive surfaces for immediate microscopic analysis. The efficiency of collection largely depends on the quality of the sampling surface to retain particles. This article compares the suitability of some traditional and new adhesive coatings to collect and retain airborne micro-organisms efficiently for subsequent fluorescence microscopy. Sacharomyces cerevisiae yeast cells were aerosolized in a test tubing system in the laboratory. Samples were collected by a round jet impactor in a defined round deposition area on glass slides coated with different adhesive materials. Particle distribution in the deposition area and collection efficiency as well as particle losses were estimated. Fluorescent staining with Mycoval and DAPI was used to quantify the amount of collected cells and cell losses due to the staining procedure. Filtration was taken as reference system. The collection efficiency of the tested adhesive surfaces ranged from 2 to 97%. Silicone sealants showed with 97% the highest collection efficiency. All particles were located in the central region of the deposition area. Cell losses due to the staining procedure were very small (<1%) and no background fluorescence was observed after DAPI staining. Even after sampling times of 45 min the collection efficiency was still above 90%. Longer collection (>50 min) resulted in increasing cell losses. Collection efficiency was little influenced by relative humidity of the air between 20% and 100%.
机译：为了对空气中的微生物进行有效采样，通常使用不同的技术，例如撞击，过滤和撞击。撞击法是最适合直接收集在胶粘剂表面上以便立即进行显微镜分析的方法。收集效率很大程度上取决于保留颗粒的采样表面的质量。本文比较了一些传统和新型胶粘剂涂层对于有效收集和保留空气中微生物以用于后续荧光显微镜检查的适用性。在实验室的测试管系统中，将酿酒酵母酵母细胞雾化。通过圆形喷射冲击器在涂覆有不同粘合剂材料的载玻片上的限定的圆形沉积区域中收集样品。估算了沉积区域中的颗粒分布，收集效率以及颗粒损失。使用Mycoval和DAPI进行的荧光染色可量化收集的细胞数量和由于染色程序而造成的细胞损失。过滤作为参考系统。被测胶粘剂表面的收集​​效率为2％至97％。硅酮密封胶显示出97％的最高收集效率。所有颗粒都位于沉积区域的中心区域。由于染色过程造成的细胞损失非常小（<1％），DAPI染色后未观察到背景荧光。即使经过45分钟的采样时间，收集效率仍高于90％。较长的收集时间（> 50分钟）导致细胞损失增加。收集效率几乎不受20％和100％之间的空气相对湿度的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号