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首页> 外文期刊>Reproduction in Domestic Animals >Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum
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Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum

机译：驴精液冷冻保存的替代品：MARE与Jenny Colostrum

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The aim of this study was to test and compare two new components in extenders for freezing donkey semen: mare colostrum and jenny colostrum. Colostrum was obtained from four mares and four jennies right after the foal's birth. Ejaculates were collected from five fertile donkeys. Sperm samples were pooled, diluted and cryopreserved in three different experimental extender groups: lactose supplemented with egg yolk extender (20%) as the control group, lactose supplemented with jenny colostrum extender (20%), and lactose supplemented with mare colostrum extender (20%). After thawing, we evaluated the sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional by HOS test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. The results demonstrated that lactose-jenny colostrum extender displayed significantly higher values (p .05) in nearly all parameters evaluated - Total Motility, Viability, HOS test, VCL, VSL, VAP, LIN, STR and WOB -, compared with mare colostrum and egg yolk extenders after thawing. In conclusion, the extender containing jenny colostrum used for donkey semen cryopreservation improved the donkey sperm quality after the freezing-thawing process.
机译：本研究的目的是测试并比较冻结驴精液的扩展器中的两个新组件：母马初乳和珍妮初乳。在小马驹的出生后，从四个母马和四个吉恩斯获得了初乳。从五个肥沃的驴收集射精。将精子样品合并，稀释和冷冻保存，在三种不同的实验扩点组中：补充蛋黄增量剂（20％）作为对照组的乳糖，乳糖补充，补充了Jenny Colostrum Extender（20％），并补充有母马初乳扩面器的乳糖（20 ％）。解冻后，我们通过计算机辅助分析，Sybr-14和碘化丙啶（PI），HOS试验和通过异硫氰酸酯与花生凝集素（FITC-PNA）和PI缀合的真主完整性的膜功能，膜功能评估了精子运动。结果表明，乳糖-JENNY Colostrum Extender在几乎所有参数中显示出显着更高的值（p，p.05） - 与Mare Colostrum相比，与Mare Colostrum相比蛋黄扩展器解冻后。总之，含有用于驴精液冷冻保存的珍妮初乳的增量剂改善了冻融过程后的驴精子质量。

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