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首页> 外文期刊>Reproduction, fertility, and development >Swim-up of tammar wallaby (Macropus eugenii) spermatozoa in Biggers, Whitter and Whittingham (BWW) medium: maximisation of sperm motility, minimisation of impairment of sperm metabolism and induction of sperm hyperactivation
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Swim-up of tammar wallaby (Macropus eugenii) spermatozoa in Biggers, Whitter and Whittingham (BWW) medium: maximisation of sperm motility, minimisation of impairment of sperm metabolism and induction of sperm hyperactivation

机译：大奖，鞭打和漂白症（BWW）中的泰马尔霍比西（Macropus Eugenii）Spermatozoa（BWW）中：精子运动的最大化，最小化精子代谢的损害和精子超动诱导

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A variety of media were compared for their ability to sustain the motility of tammar wallaby spermatozoa over an 8-h period following swim-up from coagulated semen. The study demonstrated that a modified Tyrode's solution, Biggers, Whitter and Whittingham medium (BWW) was significantly better than any of the other assessed media in supporting wallaby sperm motility. After 8 h of incubation in BWW, motility was maintained at 79.3 +/- 9.3%, with 77.0 +/- 10.4% rapid and 65.7 +/- 8.7% progressively motile spermatozoa. By contrast, motility was <10% at the same 8-h time point in all of the other media assessed. After 2 h of incubation in BWW, tammar spermatozoa consumed more oxygen than their counterparts in PBS (52.0 +/- 2.7 vs 75.0 +/- 6.6 mu L per 10(8) spermatozoa per 2 h; P < 0.001). Motility was not enhanced in any of these media by the addition of 5 mM N-acetyl-D-glucosamine, the major energy substrate in wallaby semen. However, addition of dibutyryl cAMP and pentoxifylline in BWW resulted in the extremely rapid induction of hyperactivated motility in the entire sperm population. This burst of hyperactivated motility was entirely dependent on calcium in BWW and significantly inhibited by calmidazolium, a calmodulin inhibitor. A set of computer-assisted sperm analysis parameters were identified that permitted the accurate quantification of hyperactivation rates in this species. This is the first comparative analysis of media for harvesting and incubating marsupial spermatozoa and the first record of hyperactivated motility in any marsupial species.
机译：比较各种培养基，以便在凝固精液游泳后8小时内保持侦探袋鼠精子的动力。该研究表明，改进的Tyrode的解决方案，大中，鞭打和漂白米（BWW）明显优于任何其他评估的媒体，这些介质在支持袋鼠精子运动中的任何一种。在BWW孵育8小时后，运动性保持在79.3 +/- 9.3％，77.0 +/- 10.4％急速和65.7 +/- 8.7％逐步的运动精子。相比之下，在评估所有其他媒体的同一8小时的时间点中，动力是<10％。在2小时后在BWW中孵育后，涉及PBS的PBS对应物的氧气比对手（52.0 +/- 2.7，每10（8）个单次P <0.001）消耗了更多的氧气; P <0.001）。通过加入5mM N-乙酰基-D-葡糖胺，在杂音精液中的主要能量基质中的任何一种在这些介质中没有增强动力。然而，BWW中的二丁酰基阵营和五氧化酶碱中的添加导致整个精子群体中的多动运动诱导。这种爆发的多动运动性均完全依赖于BWW中的钙，并通过钙调蛋白抑制剂的钙唑鎓显着抑制。鉴定了一组计算机辅助精子分析参数，允许准确定量该物种中的多动激活速率。这是对培养基收获和孵育碱性精子的第一种比较分析，以及任何饲养物种中的多动运动的第一记录。

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