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首页> 外文期刊>Research in Veterinary Science >Transmissible gastroenteritis virus N protein causes endoplasmic reticulum stress, up-regulates interleukin-8 expression and its subcellular localization in the porcine intestinal epithelial cell
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Transmissible gastroenteritis virus N protein causes endoplasmic reticulum stress, up-regulates interleukin-8 expression and its subcellular localization in the porcine intestinal epithelial cell

机译：传染性胃肠炎病毒N蛋白导致内质网胁迫，上高素-8表达和其在猪肠上皮细胞中的亚细胞定位

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摘要

This essay focuses on transmissible gastroenteritis virus (TGEV), which is an enteropathogenic virus related to contagious and acute diseases in suckling piglets. Previous literature suggests that the TGEV nucleocapsid protein (N) plays a significant role in viral transcriptional process, however, there is a need to examine other functions of TGEV N protein in the porcine intestinal epithelial cell (IEC) which is the target cell of TGEV. In the present study, we investigated the degradation, subcellular localisation, and function of TGEV N protein by examining its effects on cycle progression, endoplasmic reticulum (ER) stress, interleukin-8 (IL-8) expression, and cell survival. The results showed that TGEV N protein localised in the cytoplasm, inhibited IEC growth, prolonged the S-phase cell cycle by down-regulating cell cycle protein cyclin A, and was mainly degraded through the proteasome pathway. Moreover, TGEV N protein induced ER stress and activated NF-κB, which was responsible for the up-regulation of IL-8 and Bcl-2 expression. This report mainly considers the functions of TGEV N protein in IEC. To be specific, in IEC, TGEV N protein induces cell cycle prolongation at the S-phase, ER stress and up-regulates IL-8 expression. These results provide a better understanding of the functions and structural mechanisms of TGEV N protein.
机译：本文侧重于传染性胃肠炎病毒（TGEV），这是一种与哺乳病毒相关的肠致病病毒，与哺乳仔猪中的传染性和急性疾病有关。以前的文献表明，TGev核衣壳蛋白（n）在病毒转录过程中起重要作用，然而，需要检查猪肠上皮细胞（IEC）中TGEv N蛋白的其他功能，这是TGEV的靶细胞。在本研究中，我们通过检查其对循环进展，内质网（ER）应激，白细胞介素-8（IL-8）表达和细胞存活的影响来研究TGEv N蛋白的降解，亚细胞定位和功能。结果表明，通过抑制细胞周期蛋白质A，延长了IEC生长的TGEv N蛋白抑制IEC生长，延长了S相细胞周期，并且主要通过蛋白酶体途径降解。此外，TGEv N蛋白诱导ER应激和活性NF-κB，其负责IL-8和BCL-2表达的上调。本报告主要考虑IEC中TGEv N蛋白的功能。特异性，在IEC中，TGEv N蛋白在S相，ER应激和上调IL-8表达时诱导细胞周期延长。这些结果提供了更好地理解TGEV N蛋白的功能和结构机制。

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