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首页> 外文期刊>BJU international >Impedance measurements and connexin expression in human detrusor muscle from stable and unstable bladders.
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Impedance measurements and connexin expression in human detrusor muscle from stable and unstable bladders.

机译：稳定和不稳定膀胱中人类逼尿肌的阻抗测量和连接蛋白表达。

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Three of this month's Scientific Discovery papers highlight the importance of collaboration in delivering high quality scientific research. As scientific technology increases in power and cost, and specific areas of interest become more specialized, it is becoming more difficult to cover all aspects of a completeresearch story. Collaborating with other experts in the field or other fields, including industry, allows strong scientific proof to be generated for the hypothesis and aims. Building strong collaborative,inter-disciplinary, multi-institutional, international groups with academic and industrial partners is the way forward for all discovery. We look forward to publishing more of these collaborative papersin future issues of the BJU International. OBJECTIVES: To test the hypothesis that intercellular electrical coupling is altered in human detrusor smooth muscle from patients with unstable bladders. MATERIALS AND METHODS: Human detrusor biopsy samples were obtained from patients with stable and unstable bladders. Intracellular electrical impedance was measured with alternating current (20 Hz-300 kHz) across the ends of detrusor strips in an oil-gap, after correcting for extracellular space resistance. Gap junctions were identified by localization of connexins (Cx), specifically Cx45, Cx43 and Cx40 transcripts, using immunoconfocal microscopy. RESULTS: Total intracellular resistivity was greater in strips from unstable than from stable bladders (median 1246 vs 817 Omega.cm). The increase was attributed to an increase in junctional resistance; cytoplasmic resistance was unchanged. Cx43 was localized to a submucosal layer and to connective tissue; Cx40 label was confined to endothelial cells of blood vessels. Cx45 labelling was localized to detrusor bundles and appeared to be less marked in samples from unstable bladders. Semi-quantitative analysis of Northern blots showed that Cx45 expression in unstable was less than that in stable bladders. CONCLUSIONS: These data suggest that intercellular couplingis reduced in detrusor from unstable bladders. Cx45 was localized to the detrusor layer, with Cx 43 more evident in the suburothelial mucosa. Cx45 labelling was less intense in detrusor samples from unstable bladders. These results are consistent with reduced gap junction coupling in detrusor from unstable bladders.
机译：本月的《科学发现》论文中有三篇强调了协作在提供高质量科学研究中的重要性。随着科学技术的力量和成本的增加，并且特定的兴趣领域变得更加专业化，覆盖整个研究故事的各个方面变得越来越困难。与该领域或包括工业在内的其他领域的其他专家合作，可以为假设和目标生成强有力的科学证据。与学术和行业合作伙伴建立强大的协作，跨学科，多机构，国际组织是所有发现的前进之路。我们期待在北京国际大学的未来期刊上发表更多这些合作论文。目的：检验假说，膀胱不稳定患者的逼尿肌平滑肌细胞间电耦合发生改变。材料与方法：人逼尿肌活检标本取自膀胱稳定和不稳定的患者。校正细胞外空间电阻后，通过油隙中逼尿肌条两端的交流电（20 Hz-300 kHz）测量细胞内电阻抗。使用免疫共聚焦显微镜通过连接蛋白（Cx），特别是Cx45，Cx43和Cx40转录本的定位来鉴定间隙连接。结果：不稳定膀胱带的总细胞内电阻率高于稳定膀胱带（中值1246对817Ω.cm）。增加归因于结电阻的增加。细胞质抗性未改变。 Cx43定位于粘膜下层和结缔组织。 Cx40标记仅限于血管内皮细胞。 Cx45标记定位于逼尿肌束，在不稳定的膀胱样本中似乎标记较少。 Northern印迹的半定量分析表明，Cx45在不稳定膀胱中的表达低于稳定膀胱中的Cx45表达。结论：这些数据表明，不稳定膀胱的逼尿肌细胞间偶联减少。 Cx45位于逼尿肌层，Cx 43在尿路上皮下粘膜中更为明显。在不稳定膀胱的逼尿肌样本中，Cx45标记的强度较低。这些结果与不稳定膀胱中逼尿肌间隙连接的耦合减少有关。

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