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首页> 外文期刊>Critical reviews in toxicology >Albumin and hemoglobin adducts of benzo(a)pyrene in humans--analytical methods, exposure assessment, and recommendations for future directions.
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Albumin and hemoglobin adducts of benzo(a)pyrene in humans--analytical methods, exposure assessment, and recommendations for future directions.

机译：人体中苯并（a）的白蛋白和血红蛋白加合物-分析方法，接触评估和对未来方向的建议。

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摘要

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in our environment and can cause cancer. Exposure to PAHs can be assessed by protein adduct dosimetry using benzo[a]pyrene (B[a]P) as a model compound. We present an overview of analytical methods to detect B[a]P- derived protein adducts in humans, their uses in exposure assessment, and recommendations for future research. Two major methodologies, enzyme-linked immunosorbent assay (ELISA) and chemical-specific assays, could be traced in the literature but there remains limitations with both assays. ELISA is nonspecific due to cross-reactivity of the antibody with other PAHs and results are better interpreted in terms of PAH exposure. ELISA is unable to distinguish between exposed and nonexposed persons in the majority of studies. Adduct concentrations are higher by several orders of magnitude compared to those determined by chemical-specific methods. The latter methods mostly analyzed protein adducts derived by (+)-anti-B[a]P-diol epoxide. For this purpose, gas or liquid chromatography in combination with mass spectrometry or fluorescence detection were used. However, the prevalence of positive samples remained low when chemical- specific assays were used mainly due to the lack of sensitivity. Overall, data on B[a]P-derived protein adducts in humans remain inconclusive. Future research should focus on the development and standardization of a sensitive and specific method for B[a]P-derived protein adducts prior to its use in field studies. Finally, exposures of B[a]P at the workplace and via diet, a major route of exposure of the general population, can be studied. The results will contribute to the understanding of B[a]P-induced cancer and will allow for health preventive measures.
机译：多环芳烃（PAH）在我们的环境中无处不在，并可能导致癌症。可以通过蛋白质加合物剂量测定法，以苯并[a] py（B [a] P）为模型化合物，评估对PAHs的暴露。我们概述了检测人类中B [a] P衍生的蛋白质加合物的分析方法，其在暴露评估中的用途以及对未来研究的建议。文献中可以追溯到两种主要的方法学，即酶联免疫吸附测定（ELISA）和化学特异性测定，但是这两种测定仍然存在局限性。 ELISA由于抗体与其他PAH的交叉反应性而具有非特异性，因此在PAH暴露方面可以更好地解释结果。在大多数研究中，ELISA无法区分接触者和未接触者。与通过化学方法确定的加合物浓度相比，加合物的浓度要高几个数量级。后一种方法主要分析了由（+）-抗-B [a] P-二醇环氧化物衍生的蛋白质加合物。为此，使用了气相色谱或液相色谱结合质谱或荧光检测的方法。但是，使用化学特异分析时阳性样品的患病率仍然很低，这主要是由于缺乏敏感性。总体而言，关于人类中B [a] P衍生的蛋白加合物的数据尚无定论。未来的研究应将重点放在针对B [a] P衍生的蛋白质加合物的灵敏和特异性方法的开发和标准化上，然后再将其用于现场研究。最后，可以研究在工作场所和饮食中B [a] P的暴露，这是普通人群暴露的主要途径。研究结果将有助于人们了解B [a] P诱发的癌症，并将采取健康预防措施。

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